| 准噶尔雅罗鱼染色体核型及带型的初步研究 |
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| 引用本文： | 王佳君,胡文革,孔磊.准噶尔雅罗鱼染色体核型及带型的初步研究[J].动物学杂志,2010,45(6):120-126. |
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| 作者姓名： | 王佳君 胡文革 孔磊 |
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| 作者单位： | 石河子大学生命科学学院,新疆石河子,832003 |
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| 基金项目： | 国家自然科学基金项目(No.30860215) |
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| 摘 要： | 以肾细胞作材料,采用秋水仙素-低渗-空气干燥法、Ag-NORs、C-带和G-带显带技术对准噶尔雅罗鱼(Leuciscus merzbacheri)染色体进行了研究。结果表明:(1)准噶尔雅罗鱼2n=50,核型组成为18m+14sm+6st+12t,NF=82,没有异型性染色体分化。(2)Ag-NORs的数目在不同的细胞中表现出多态性,数目为1～2个,出现1个Ag-NORs的频率最低(10%),出现2个的频率最高(70%);Ag-NORs主要出现在m1对和m4对同源染色体上;未发现有Ag-NORs联合的现象。(3)准噶尔雅罗鱼的染色体均呈现C-带阳性,可分为着丝粒C-带和端粒C-带。(4)同源染色体上G-带带纹基本一致,其带纹在每对染色体上的数目及分布具有明显特征性。
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| 关 键 词： | 准噶尔雅罗鱼 核型 Ag-NORs C-带 G-带 |
| 收稿时间： | 2010/5/24 0:00:00 |
| 修稿时间： | 2010/9/20 0:00:00 |
Karyotype and Banding Pattern of Leuciscus merzbacheri |
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| WANG Jia-Jun,HU Wen-Ge and KONG Lei.Karyotype and Banding Pattern of Leuciscus merzbacheri[J].Chinese Journal of Zoology,2010,45(6):120-126. |
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| Authors: | WANG Jia-Jun HU Wen-Ge and KONG Lei |
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| Institution: | WANG Jia-Jun HU Wen-Ge KONG Lei(College of Life Sciences,Shihezi University,Shihezi,Xinjiang 832003,China) |
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| Abstract: | The chromosome spreading of metaphase kidney cells was conducted by colchicine-low concentration-air drying technique in Leuciscus merzbacheri,and Ag-NORs,C-band and G-band were analyzed in this paper.L.merzbacheri had a diploid chromosome number of 2n = 50,its karyotype formula was 18m + 14sm + 6st + 12t,NF = 82,and sex chromosome was not found.The Ag-NORs polymorphisms were individually specific,1-2 in number,and the frequency of single Ag-NORs was the lowest(10%),while the frequency of 2 Ag-NORs was the ... |
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| Keywords: | Leuciscus merzbacheri Karyotype Ag-NORs C-band G-band |
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