重庆发现云南小狭口蛙及其系统发育关系

2021年5月在重庆武隆仙女山国家森林公园采集到3号小狭口蛙标本，经形态学及分子生物学鉴定为云南小狭口蛙（Glyphoglossus yunnanensis），属重庆市两栖动物分布新记录种。本文给出了这3号标本的17项外形特征测量值，并描述了其生境特征。对10个不同地理种群的云南小狭口蛙种群相关16S rRNA基因片段构建最大似然树表明，重庆武隆标本与云南小狭口蛙产地标本序列聚在一起，且其间的遗传距离为0.6%，远远小于小狭口蛙属物种之间的遗传距离。重庆武隆的这一分布点，使得对云南小狭口蛙在我国分布的认知往东北延伸了超过350 km。云南小狭口蛙在重庆武隆的分布新记录能帮助理解该物种潜在分布区以及谱系地理。

Discovery of Yunnan Small Narrow-mouthed Frogs in Chongqing and Their Phylogenetic Relationships

Three specimens of Glyphoglossus were collected from Chongqing Wulong Fairy Mountain National Forest Park in May 2021. The specimens were identified as G. yunnanensis (Amphibia, Anura, Microhylidae) by morphological and molecular data, which is a new record of this species in Chongqing municipality. Seventeen morphological characteristics of the specimens were measured (Table 2) and their habitats (Fig. 1) were also described. The construction of maximum likelihood trees for 16S rRNA gene fragments associated with 10 different geographic populations of G. yunnanensis populations showed that the sequences of specimens from Wulong, Chongqing, clustered with those from G. yunnanensis frog locality, and the genetic distance between them was 0.6%, much smaller than that between species of the genus Glyphoglossus (Table 2 and Fig. 2). This new locality in Wulong, Chongqing extends the distribution area of this species northeastward for more than 350 km in China (Fig. 3). The new record of G. yunnanensis in Chongqing will play an important role in understanding the potential distribution area and pedigree geography of this species.