饲料中添加菊粉对暗纹东方鲀幼鱼生长、 消化及非特异性免疫能力的影响

为探讨菊粉对暗纹东方鲀（Takifugu obscures）幼鱼生长、消化及免疫能力的影响，利用单因素实验设计，选取640尾暗纹东方鲀，体重（6.97 ± 1.32）g，随机分为4组，每组设4个重复，在日粮中添加不同浓度的菊粉（0%、0.25%、0.5%、1%），连续投喂8周后测定鱼体生长、肌肉营养成分、肠道消化酶和肝非特异性免疫酶活性。结果显示，各菊粉添加组的生长指标无显著性差异（P > 0.05）；0.25%组肌肉的粗脂肪含量（0.87%）显著高于0.5%组（0.82%）和1%组（0.76%）；0.25%组肌肉的粗蛋白含量（18.75%）显著高于1%组（18.50%），与对照组（18.62%）和0.5%组（18.60%）无显著差异。暗纹东方鲀幼鱼肠道组织的胃蛋白酶、胰蛋白酶、脂肪酶和淀粉酶活性均随着菊粉添加量的增加而呈逐渐上升的趋势。0.5%菊粉添加组肝组织的谷胱甘肽过氧化物酶和总超氧化物歧化酶活性极显著（P < 0.01）高于其余处理组；0.5%组的过氧化氢酶活性也极显著（P < 0.01）高于0.25%和1%组；丙二醛含量随着菊粉添加量的增加而呈递减的趋势。因此，菊粉添加量以0.5%为宜，对暗纹东方鲀幼鱼的生长无显著影响，但对其消化酶活性和非特异性免疫酶活性均具有促进作用。

Effect of Supplemental Inulin in Dietary on the Growth, Digestive Enzyme and Nonspecific Immunity in Juvenile Takifugu obscures

To study effect of dietary Inulin on the growth, digestive function, and immunity of juvenile Takifugu obscures, a total of 640 juvenile T. obscures (initial body weight of 6.97 ± 1.32 g) were randomly divided into 4 groups with 4 replicates and data were analyzed by single factor test. Four groups were fed diets with 0% (control group), 0.25%, 0.5% and 1% inulin, respectively. After rearing 8 weeks, the growth, nutritive composition, activities of digestive enzymes, nonspecific immunity enzyme were investigated. The supplementation of Inulin had no significant effect on growth index of juvenile T. obscures (P > 0.05). The crude fat content in the white muscle of 0.25% group (0.87%) was significantly higher than that of 0.5% group (0.82%) and 1% group (0.76%), and the crude protein content in the white muscle of 0.25% group (18.75%) was significantly higher than that of 1% group (18.50%), but showed no significant difference compared with the control group (18.62%) and 0.5% group (18.60%); The activities of pepsin, trypsin, lipase and amylase in intestine were increased with the increase of inulin addition. The activities of glutathione peroxidase and superoxide dismutase in the liver of 0.5% group were significantly higher than in the rest three groups, and the difference was very significant (P < 0.01). The activity of catalase in intestine of 0.5% group was very significantly higher (P < 0.01) than that of 0.25% or 1% group, while the malondialdehyde content was decreased with the increase of inulin addition. Therefore, the added amount of inulin should be 0.5%, a concentration that that cannot promote the growth significantly, but improve the activities of digestive enzymes and nonspecific immunity enzymes of juvenile T. obscures.