塑料袋包装充氧运输胁迫对四指马鲅幼鱼抗氧化系统的影响及抗应激剂的作用

为探究塑料袋包装充氧运输胁迫对四指马鲅(Eleutheronema tetradactylum)幼鱼肝、肌肉抗氧化系统的影响以及抗应激剂的生理作用。本研究设置了未经运输的幼鱼30尾作为对照组、不添加任何抗应激剂运输的幼鱼90尾作为空白组、添加维生素C(VC)运输的幼鱼90尾作为维生素C组以及添加谷氨酰胺(Gln)运输的幼鱼90尾作为谷氨酰胺组,在运输实验进行的2 h、6 h以及9 h采样,不同时间点每组各取30尾幼鱼分别采集肝、肌肉样品进行相关指标的测定。测定项目包括:超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、还原型谷胱甘肽(GSH)、丙二醛(MDA)以及总抗氧化能力(T-AOC)。结果显示,运输胁迫使空白组肝和肌肉中SOD活性最终显著(P0.05)高于对照组,而维生素C组和谷氨酰胺组由于抗应激剂的存在而使其最终活性低于对照组;运输胁迫使空白组和维生素C组肝以及肌肉中CAT活性显著(P0.05)升高,最终其活性均显著(P0.05)高于对照组,而谷氨酰胺组最终值与对照组无显著差异(P0.05);运输胁迫使空白组肝和肌肉MDA含量均显著(P0.05)升高,而抗应激剂维生素C和谷氨酰胺明显缓解了这一现象;GSH含量在空白组、维生素C组和谷氨酰胺组的肝与肌肉中均出现了不同的变化趋势,可能与这两种组织所承担的生理功能不同有关;运输胁迫后,空白组肝与肌肉T-AOC值均显著(P0.05)高于对照组,而维生素C组和谷氨酰胺组最终则略高于或等于对照组。本研究分析了运输胁迫下四指马鲅幼鱼抗氧化系统变化规律以及抗应激的作用效果,旨在为其养殖生产提供参考依据。

Effects of Packing in Plastic Bags and Oxygenating Transport Stress on Antioxidant System and Effects of Anti-Stress Agent in Juvenile Eleutheronema tetradactylum

In order to explore the effects of packing transport stress on antioxidant system in liver and muscle and effects of anti-stress agent in juvenile Eleutheronema tetradactylum,various groups including control group (without transportation),blank group (transported but without anti-stress agent),vitamin C group (vitamin C was added as an anti-stress agent) and glutamine group (glutamine was added as an anti-stress agent) were set in this study.Liver and muscle samples of juvenile E.tetradactylum were collected at 2 h,6 h,and 9 h of transport stress to determine the related indexes.Tissue samples were preserved in liquid nitrogen immediately.The samples were added with normal saline according at a ratio of 1 ∶ 9,then they were made into 10％ tissue homogenate after crushing and centrifugation.Measured items included superoxide dismutase (SOD),catalase (CAT),malondialdehyde (MDA),glutathione (GSH),total antioxidant capacity (T-AOC) in liver and muscle.Results showed that transport stress led to rapid increase in SOD activity in liver and muscle in blank group,however,SOD activity was significantly (P ＜ 0.05) lower than that of the control group in vitamin C group and glutamine group,while there was no significant difference (P ＞ 0.05) between the latter two groups (Fig.1).Transport stress led to significant increase in the activity of CAT (P ＜ 0.05) in liver and muscle in blank group,although slightly decreased later,the activity values were significantly higher than those in control group (P ＜ 0.05) finally;however,these values in vitamin C group and glutamine group were not significantly different compared with the control group (P ＞ 0.05) or slightly higher than that in control group (Fig.2).Transport stress led to significant increase in the content of MDA (P ＜ 0.05) in muscle and liver in blank group,but this result was alleviated significantly in vitamin C group and glutamine groups (Fig.3).The contents of GSH showed different change trends between liver and muscle in different groups,and this might be related to their different physiological functions (Fig.4).The values of T-AOC in liver and muscle were significantly higher in blank group than that in control group (P ＜ 0.05),while the values were slightly higher in vitamin C group and glutamine groups than that in control group (Fig.5).The analysis of antioxidant system of juvenile E.tetradactylum under the transport stress and the effects of anti-stress agent will provide reference for promoting artificial breeding ofE.tetradactylum.