大鼠短间隔连续部分肝切除上调表达基因的克隆与分析 * |
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引用本文: | 李玉昌,徐存拴,林俊堂,张会勇,李文强,魏明旭,张云汉.大鼠短间隔连续部分肝切除上调表达基因的克隆与分析 *[J].动物学杂志,2003,38(1):22-27. |
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作者姓名: | 李玉昌 徐存拴 林俊堂 张会勇 李文强 魏明旭 张云汉 |
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作者单位: | 1. 河南师范大学生命科学学院,新乡,453002 2. 河南省肿瘤病理重点实验室,郑州,450052 |
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基金项目: | 国家自然科学基金,河南省科技攻关项目,39970362,0122031990,, |
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摘 要: | 以短间隔连续部分肝切除112h为试验方,以0h对照为驱动方,应用抑制性消减杂交技术构建了高效率的正向消减cDNA文库,从中随机挑取的50个克隆中有45个包含了100~350bp插入片段,对这些片段进行测序后经GenBank blast同源性检索,表明8个片段均为未知新序列。大鼠短间隔连续部分肝切除后肝再生cDNA正向消减文库的建立和未知的上调表达基因片段的克隆为研究肝再生的分子机理奠定了基础。
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关 键 词: | 短间隔连续部分肝切除 抑制性消减杂交 肝再生 大鼠 上调表达基因 基因克隆 |
文章编号: | 0250-3263(2003)01-22-05 |
Screening and Identification of Up-regulated Expressed Genes by Suppression Subtractive Hybridization in Rat Liver Regeneration after Short Interval Successive Partial Hepatectomy |
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Abstract: | In this study,suppression subtractive hybridiation(SSH) was carried out using normal rat liver tissue as the driver and the 112?h tissue following short interval successive partial hepatectomy(SISPH) as the tester to establish a highly efficient forward subtractive cDNA library.After scree\|ning,45 of 50 clones derived from the cDNA library were inserted into 100-450?bp cDNA fragments.Among these up regulated clones,8 cDNA fragments were from unknown sequences(GenBank accession number:BM403207,BM403208,BM403209,BM403210,BM285361,BM285365,BM285369,BM2853). |
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Keywords: | Short interval successive partial hepatectomy Suppression subtractive hybridization Liver regeneration Rat |
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