蛇岛蝮蛇消化道5-羟色胺细胞的形态与分布

采用ABC(avidin-biotin-peroxidase complex)免疫组织化学方法,观察蛇岛蝮蛇(Gloydius shedaoensis)消化道内5-羟色胺(5-HT)免疫阳性内分泌细胞的分布及形态.结果显示,5-羟色胺细胞从食管到直肠各段均有分布.细胞分布密度呈波浪式,其中胃贲门部分布密度最高(7.15±2.38),直肠部次之(4.55±3.14),食管部最低(1.2±0.71).5-HT阳性细胞广泛分布于消化道上皮细胞之间、上皮基部、腺泡上皮细胞之间以及固有膜内.形态多样,呈圆形、锥体形、梭形等.分析认为蛇岛蝮蛇消化道5-HT细胞具有内、外分泌两种作用途径,并且其密度分布可能与其食性、生存环境有关.

The Morphology and Distribution of 5-HT Cells in Digestive Tract of Gloydius shedaoensis

Immunohistochemical localization and morphology of 5-Hydroxytryptamine immunoreactive cells (5-HT cells) in the digestive tract of Gloydius shedaoensis have been investigated by using the ABC(avidin-biotin-peroxidase complex)immunostaining technique. The 5-HT positive immunoreactive cells could be observed throughout the digestive tract, from esophagus to rectum. The density curve appeared wavelike and the cell density (number/field of vision) was the highest in the cardiac part of stomach (7.15±2.38), followed by the pylorica part of the rectum (4.55±3.14), and the lowest cell density was found in the esophagus (1.2±0.71).The 5-HT cells distributed at the bottom of epithelial cells, between epithelial cells, between glandular epithelial cells or in the lamina propria. The 5-HT cells had different shapes including round, shuttle-like, tapering and other shapes. We suggest that 5-HT positive immunoreactive cells in the digestive tract of G.shedaoensis should have both endocrine and exocrine functions, and the distributive density is related to its feeding habit and life environment in G.shedaoensis.