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不同海拔高度大血藤群体遗传多样性的RAPD分析及其与环境因子的相关性
引用本文:李钧敏,金则新,钟章成.不同海拔高度大血藤群体遗传多样性的RAPD分析及其与环境因子的相关性[J].生态学报,2004,24(3):567-573.
作者姓名:李钧敏  金则新  钟章成
作者单位:1. 台州学院生物系,浙江,临海,317000
2. 西南师范大学生命科学学院,重庆,北碚,400715
基金项目:国家自然科学基金资助项目 ( 39870 1 6 0 )~~
摘    要:利用 RAPD技术分析了分布于浙江省天台山 3个不同海拔高度的天然大血藤群体的遗传多样性、遗传分化以及与环境因子的相关性。 13种随机引物在 3 6株个体中共检测到 88个可重复的位点 ,其中多态位点 74个 ,总多态位点百分率为84.0 9% ,大血藤具有丰富的遗传多样性。 Shannon信息指数显示的遗传多样性以海拔 950 m的群体为最高 ,其次是海拔 73 0 m的群体 ,最低的是海拔 52 0 m的群体 ;群体内的遗传多样性占总遗传多样性的 43 .68% ,群体间的遗传多样性占 56.3 2 %。 Nei指数估计大血藤群体间的遗传分化系数为 0 .540 6,大血藤群体间的基因流很低。大血藤海拔 73 0 m群体与海拔 52 0 m群体的遗传相似度较高 ,海拔 950 m群体与其它两群体的遗传相似度较低。大血藤群体内的遗传多样性与土壤总氮呈极显著的正相关。

关 键 词:大血藤  RAPD  遗传变异  海拔高度  环境因子
文章编号:1000-0933(2004)03-0567-07
收稿时间:2/7/2003 12:00:00 AM
修稿时间:2003/9/18 0:00:00

RAPD analysis of genetic diversity of Sargentodoxa cuneata at different altitude and the influence of environmental factors
LI Junmin,JIN Zexin and ZHONG Zhangcheng.RAPD analysis of genetic diversity of Sargentodoxa cuneata at different altitude and the influence of environmental factors[J].Acta Ecologica Sinica,2004,24(3):567-573.
Authors:LI Junmin  JIN Zexin and ZHONG Zhangcheng
Institution:Department of Biology; Taizhou University; Linhai; Zhejiang; China
Abstract:The genetic diversity within populations and genetic differentiation among populations of Sargentodoxa cuneata at three different altitudes in Tiantai mountain of Zhejiang Province were analyzed by random amplified polymorphic DNA technique. To explore how S. cuneata fits environment, the relationships between the genetic diversity and the environmental factors were analyzed. 13 random primers were selected in the amplification and 88 repetitive loci with 74 polymorphic loci were produced. The total average percentage of polymorphic loci was 84.09%. Among three populations of S. cuneata, the populations at the altitude of 950m showed the most bands and the percentage of polymorphic loci was 39.77%. The percentage of polymorphic loci of the population at the altitude of 730m was 32.95% and took the second place. The population at the altitude of 520m showed the lowest bands and the percentage of polymorphic loci was 31.82%. All of them were distinctively lower than the percentage of total polymorphic loci. Different ecological microhabitat could lead to the difference of the genetic structure. Shannon index of phenotypic diversity shows that the highest genetic diversity occured in the population at the altitude of 950m. The second one occurred in the population at the altitude of 730m and the lowest genetic diversity occured in the population at the altitude of 520m. But the difference between the population at the altitude of 730m and the population at the altitude of 520m was relatively small. The average genetic diversity within populations of S. cuneata accounted for 43.68% of the total genetic diversity while the genetic diversity among populations accounted for 56.32%. All of these show that the genetic variation mainly existed among the populations of S. cuneata. The genetic diversity of S. cuneata estimated with Nei index was lower than that estimated with Shannon index of phenotypic diversity. But the variation trend of the genetic diversity within three populations, as indicated by these two indice, was generally consistent. The genetic differentiation among three populations, as estimated by Nei index, was 0.5406. The distribution of the genetic variation was generally consistent, i.e. the genetic variation among populations was relatively high and that within populations was relatively low. The gene flow estimated from Gst was only 0.4018 and it indicates that gene flow among populations of S. cuneata was relatively low. The genetic identity between the population at the altitude of 950m and the population at the altitude of 730m was the lowest while that between the population at the altitude of 730m and the population at the altitude of 520m was the highest. The genetic distance matrix was calculated using the method published by Nei and the clustering analysis was made using the unweighted pair group method with arithmetic mean (UPGMA). The population at the altitude of 520m clustered with population at the altitude of 730m, and then they clustered with population at the altitude of 950m. The correlation analysis showed that the genetic diversity within populations was significantly positively related with the soil total nitrogen. It indicates that nitrogen was important in the maintenance of the genetic diversity of S. cuneata.
Keywords:Sargentodoxa cuneata  RAPD  genetic variation    altitude  environmental factors
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