几种牧草幼苗对冷蒿茎叶水浸提液化感作用的生理响应

采用沙培法对草木樨、披碱草、冰草和羊草进行幼苗生长试验,研究了不同浓度冷蒿茎叶水浸提液处理对4种牧草幼苗生长、根系活力、叶绿素含量以及抗氧化保护酶活性的影响,并采用气相色谱-质谱(GC-MS)联用技术对冷蒿茎叶水浸提液的化学成分进行了分析。结果表明:冷蒿茎叶水浸提液对草木樨、披碱草、冰草和羊草4种牧草幼苗生长有明显的影响,这种影响效应与牧草的种类及冷蒿茎叶水浸提液浓度显著相关。其中,25mgDw·mL-1的冷蒿茎叶水浸提液均表现出对4种牧草幼苗生长指标有极显著的抑制作用(P0.01),4种牧草幼苗根系活力分别降低了46.7%、65.9%、59.3%和66.7%,草木樨、披碱草和羊草幼苗叶绿素含量分别下降了47.3%、56.6%和46.7%。当冷蒿茎叶水浸提液浓度为10mgDw·mL-1时,对4种牧草幼苗体内超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性有显著的促进作用;当浓度为25mgDw·mL-1时,对4种牧草幼苗体内SOD、POD和CAT活性有显著的抑制作用,同时使幼苗体内丙二醛(MDA)的含量显著增加。冷蒿茎叶水浸提液有30种化感类次生代谢化合物,主要成分是樟脑(27.6%)、龙脑(10.1%)、蓍草苦素(9.8%)、桉树脑(8.7%)、喇叭烯醇(4.1%)和对-1-薄荷烯-4-醇(4.1%),占总量的60%以上。

Physiological responses to allelopathy of aquatic stem and leaf extract of Artemisia frigida in seedling of several pasture plants

In order to understand the interaction of Artemisia frigida with other pasture plants,we studied the effects of the aquatic extract from stem and leaves of A.frigida on the growth,root activity,chlorophyll content,and antioxidant defense system in seedlings of Melilotus officinalis Lam.,Elymus dahuricus Turcz.,Agropyron cristatum L.and Leymus chinensis by using sand culture method,and analyzed the chemical components of the extract using GC-MS.Results showed that the aquatic stem and leaf extract of A.frigi...