利用AFLP分子标记探讨蜡梅种质资源的遗传多样性

利用AFLP分子标记技术,对中国蜡梅种质资源7个野生种群的遗传多样性进行了研究。利用筛选出的3对引物,共扩增出253条谱带,其中218条多态带,多态位点占86.17% ;种群间的基因分化系数为0.2906,说明蜡梅基因多样性主要存在于种群内;种群总的Nei s基因多样性指数为0.2933,Shannon信息多态性指数为0.4487,蜡梅总的遗传多样性水平较高。蜡梅不同种群遗传多样性水平差异较大,种群多态位点百分率在65.44% ～87.16%之间,Nei s基因多样性指数为0.1653 ～0.4012,Shannon信息多态性指数为0.3132 ～0.5603。神农架种群(SN)和保康种群(BK)的遗传多样性水平较高。用NTSYS2.01版软件对样品进行UPGMA聚类分析,结果7个种群并没有按地理距离进行聚类。最后提出要对各蜡梅野生群体采取相应的迁地和就地保护措施。

Genetic diversity of germplasm resources of Chimonanthus praecox(L.)Link based on AFLP marker

Levels of genetic diversity within and among seven wild populations of Chimonanthus praecox were analyzed using amplified fragment length polymorphism(AFLP) markers.253 bands were amplified by three informative and reliable primers screened,of which 218(86.17%) were polymorphic.,Genetic differentiation coefficient among populations was 0.2906,which indicated genetic differentiation mainly occurred within populations.The level of genetic diversity of Ch.praecox was relatively high in terms of Nei's gene diversity index(0.2933) and Shannon's information index(0.4487).Genetic diversity difference among populations was relatively high based on the percentage of polymorphic loci(PPL) ranged from 65.44% to 87.16%,Nei's gene diversity index ranged from 0.1653 to 0.4012,and shannon's information index ranged from 0.3132 to 0.5603.The genetic diversity levels of population Shennongjia(SN) and Baokang(BK) were higher than other populations investigated.UPGMA cluster analysis was performed based on Nei and Li genetic similarity with soft NTYSIS2.01,however,the clades formed including seven populations.differed form cluster by geographic distance.Finally ex situ and in situ conservation of wild Ch.Praecox populations were suggested.