稻、麦秸秆对球形棕囊藻(Phaeocystis globosa)生长的抑制作用

考查了秸秆及其浸出液对球形棕囊藻(Phaeocystis globosa)生长的影响,分析了秸秆附着微生物及秸秆物理吸附作用对秸秆抑藻活性的影响,观察了稻杆浸出液对藻细胞形态的影响。结果显示,一定量的物理破碎稻杆、麦杆及其浸出液均可有效抑制棕囊藻的生长,灭菌与未灭菌秸杆之间抑藻效果相差不大。不同条件处理的秸杆浸出液总酚含量与抑藻作用间均存在很大差异,但两者之间并无明显相关性。原子力显微镜观察发现,秸秆处理组细胞膜破裂、细胞塌陷,结构不完整,细胞粗糙度明显增加。这些结果提示微生物和物理吸附对秸杆抑藻作用的贡献很小,秸秆中存在的或降解产生的抑藻活性成分是秸秆抑藻的主要原因;除了酚酸类物质外,秸杆中可能存在其他可以显著抑制藻类生长的物质;秸秆通过破坏藻细胞的膜结构,导致藻细胞出现空洞,细胞内容物流出,从而抑制或杀灭藻细胞。

Inhibitory effects of rice straw and barely straw on the growth on Phaeocystis globosa

The effects of finely-chopped straws and aqueous extracts from barely straw and rice straw on the growth of Phaeocystis globosa were observed.The roles of microorganism and possible adsorption of chopped straws in the inhibition were assessed.The morphological changes in algal cells induced by straw extract were observed by AFM.Both finely chopped straws and aqueous extracts of straws were showed to have inhibitory effect on Phaeocystis globosa.There were little difference in algistatic action between asepsis straw and rude straw,suggesting that some antialgal compounds from straws might be responsible for the inhibition,and that physical adsorption and microorganisms from straw had little effect on the inhibition.The total phenol levels and antialgal action of the extracts obtained under various conditions were different,but no direct relationship between phenol level and antialgal action were found,which indicated that the antialgal compound or algaecide other than phenol,existed in extracts.Morphological changes such as increase in cellular roughness,shrink of cell structure and rupture of cell membrane,etc.were observed by AFM in the algal cell treated with extracts.From the results,it was suggested that barely straw and rice straw had certain inhibitory effect on Phaeocystis globosa and some antialgal compounds besides phenol might be responsible for the inhibition.Damages of cell membrane might be cause of the death of the algal cell induced by straw extract.