| 菲律宾蛤仔大连群体不同世代的遗传多样性 |
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| 引用本文： | 虞志飞,闫喜武,杨霏,王金海,张跃环,杨凤,张国范.菲律宾蛤仔大连群体不同世代的遗传多样性[J].生态学报,2011,31(15):4199-4206. |
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| 作者姓名： | 虞志飞 闫喜武 杨霏 王金海 张跃环 杨凤 张国范 |
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| 作者单位： | 1. 大连海洋大学生命科学与技术学院,辽宁,大连,116023
2. 中国科学院海洋研究所,山东,青岛,266071 |
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| 基金项目： | 国家"十二五"科技支撑计划(2011BAD16B14);农业行业科研专项(200903007) |
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| 摘 要： | 采用12对有效微卫星引物对大连群体菲律宾蛤仔连续4个选育世代(F1、F2、F3、F4)的144个个体进行了遗传多样性分析。结果表明:共获121个等位基因,每个位点的等位基因数在2-6个不等,其大小在101-273 bp之间;各个世代平均等位基因数在3.75-4.58,平均观测杂合度在0.3391-0.3860之间。从F-检验结果上看,所有世代内有2个位点遗传分化较弱,8个位点遗传分化中等,2个位点遗传分化较大;配对比较Fst值(0.05-0.15)表明4个世代群体间遗传分化程度中等。Fis值表明有2个世代位点杂合度处于过剩状态;但对连续4个世代而言,每个世代均表现出一定程度的杂合子缺失。随着世代连续选育的进行,Nei氏遗传相似性逐渐减小(0.8203-0.8107-0.8031);遗传距离逐渐增大(0.1918-0.2099-0.2129);不同世代群体间遗传相似性系数为0.7873-0.8685,遗传距离为0.141-0.2391。4个世代平均PIC值为0.5055,表明选育后代遗传多样性较好,还有较大的选育潜力,可以继续进行上选。
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| 关 键 词： | 菲律宾蛤仔 微卫星 遗传多样性 遗传分化 |
| 收稿时间： | 2010/12/1 0:00:00 |
| 修稿时间： | 2011/4/26 0:00:00 |
Genetic diversity of different generations of the Dalian population of Manila clam Ruditapes philippinarum through selective breeding |
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| YU Zhifei,YAN Xiwu,YANG Fei,WANG Jinhai,ZHANG Yuehuan,YANG Feng and ZHANG Guofan.Genetic diversity of different generations of the Dalian population of Manila clam Ruditapes philippinarum through selective breeding[J].Acta Ecologica Sinica,2011,31(15):4199-4206. |
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| Authors: | YU Zhifei YAN Xiwu YANG Fei WANG Jinhai ZHANG Yuehuan YANG Feng and ZHANG Guofan |
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| Institution: | The Institute of Life Science and Technology, Dalian Ocean University, Dalian 116023, China;The Institute of Life Science and Technology, Dalian Ocean University, Dalian 116023, China;The Institute of Life Science and Technology, Dalian Ocean University, Dalian 116023, China;The Institute of Life Science and Technology, Dalian Ocean University, Dalian 116023, China;The Institute of Life Science and Technology, Dalian Ocean University, Dalian 116023, China;The Institute of Life Science and Technology, Dalian Ocean University, Dalian 116023, China;Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China |
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| Abstract: | The present study examined the genetic structure and variation among different generations of Manila clam Ruditapes philippinarum obtained through selective breeding by using 12 microsatellite markers.Four consecutive generations(F1,F2,F3,and F4) were selected with 10% selection intensity from a wild population from Shihe,Dalian(Liaoning Province,China).Thirty six individuals were sampled from each generation.Among the 12 microsatellite loci,121 alleles were detected.The number of alleles on each locus vari... |
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| Keywords: | Ruditapes philippinarum microsatellite genetic diversity genetic differentiation |
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