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| 小花棘豆(Oxytropis glabra DC.)内生真菌的培养与鉴定 | |
| 引用本文： | 卢萍,Dennis Chil,赵萌莉,Dale R Gardener,吕桂芬,韩国栋.小花棘豆(Oxytropis glabra DC.)内生真菌的培养与鉴定[J].生态学报,2009,29(1):53-58. |
| 作者姓名： | 卢萍 Dennis Chil 赵萌莉 Dale R Gardener 吕桂芬 韩国栋 |
| 作者单位： | 1. 内蒙古师范大学生命科学与技术学院,呼和浩特,010022 2. 美国科罗拉多州立大学自然资源学院 3. 内蒙古农业大学生态环境学院,呼和浩特,010018 4. 美国农业部有毒植物实验室 |
| 基金项目： | 国家自然科学基金，内蒙古自治区自然科学基金，内蒙古师范大学自然科学基金 |
| 摘 要： | 小花棘豆(Oxytropis glabra DC.)是内蒙古草原上的重要毒草,实验结合微生物学和分子生物学手段进行了其内生真菌研究.结果表明:体外培养的小花棘豆内生真菌生长缓慢,呈圆形、隆起、边缘整齐、辐射状生长的白色菌落,后菌体分泌黑褐色的色素物质,分生孢子近圆柱形,有粗且比孢子壁厚的暗色横隔膜,隔膜数1～5个.10个菌株的5.8S rDNA/ITS序列与内生真菌Embellisia sp.L12株的序列高度相似.推测该内生真菌属于Embellisia. |
| 关 键 词： | 小花棘豆 内生真菌 分生孢子 |
| 收稿时间： | 6/6/2008 12:00:00 AM |
| 修稿时间： | 2008/11/17 0:00:00 |
Culture and identification of endophytic fungi from Oxytropis glabra DC. |
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| LU Ping,Dennis Child,ZHAO Meng-Li,Dale R Gardener,LU Gui-Fen,HAN Guo-Dong.Culture and identification of endophytic fungi from Oxytropis glabra DC.[J].Acta Ecologica Sinica,2009,29(1):53-58. | |
| Authors: | LU Ping Dennis Child ZHAO Meng-Li Dale R Gardener LU Gui-Fen HAN Guo-Dong |
| Abstract: | The Oxytropis glabra DC. is an important poisonous plant species in Inner Mongolia steppe. In this research the endophytic fungi from O.glabra DC. were studied both by microbiological and molecular biological techniques. The results showed as follows: The in vitro cultured endophytic fungi displayed round, raised, with uniform margin and radial growth colonies which grew slowly. Later a black brown pigment was secreted in each colony. The conidia were near cylindraceous with dark transverse septa thicker than conidial walls. The septa numbers varied from 1 to 5. The sequences of 5.8S rDNA/ITS from 10 strains of O.glabra DC. were highly similar to that of Embellisia sp.L12, which indicated the endophytes might belong to Embellisia. |
| Keywords: | 5 8SrDNA/ITS |
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