草履蚧不同寄主和地理种群遗传分化的RAPD分析

应用随机扩增多态性DNA(RAPD)技术对草履蚧保定、石家庄、邯郸16个不同寄主地理种群遗传多样性和种群分化进行研究,结果显示4个RAPD引物共扩增出41个多态性位点,多态位点比率为100%。遗传距离指数在0.701—0.4360,平均为0.2395。其中以邯郸枫杨和邯郸垂丝海棠为寄主的草履蚧种群遗传距离最小(0.0701);以石家庄紫叶李和邯郸木槿为寄主的种群遗传距离最大(0.4360)。遗传一致度系数在0.6466—0.9290。说明草履蚧不同种群遗传多样性丰富并存在遗传差异。聚类分析结果表明草履蚧种群遗传多样性同时受到寄主和地理因素的双重影响,且不同寄主草履蚧种群已产生明显的遗传分化。

RAPD analysis of genetic differentiation in the different hosts and geographic populations of Drosicha corpulenta

The genetic diversity and population differentiation among geographic populations of 16 different Drosicha corpulenta host collected from Baoding, Shijiazhuang, and Handan in Hebei were analyzed by RAPD. The results showed that four RAPD primers were amplified out of 41 polymorphism loci, and that the percent of polymorphic loci was 100%. The genetic distance index of the populations ranged from 0.0701 to 0.4360 and the average value was 0.2395. The genetic distance index for D. corpulenta populations on Pterocarya stenoptera and Malus halliana(Voss.) Koehne in Handan was the smallest(0.0701). The genetic distance index for D. corpulenta populations on Prunus cerasifera in Shijiazhuang and Hibiscus syriacus in Handan was the largest of all, and the similarity coefficients ranged from 0.6466 to 0.9290.The genetic diversity of the different D. corpulenta populations was classified as abundant, and genetic differences were found. According to the results of cluster analysis, the genetic diversity of D. corpulenta populations was affected by both host and geographic location, and the different host populations of D. corpulenta showed obvious genetic differentiation.