| 应用单克隆抗体评价拟环纹豹蛛对褐飞虱的控制作用 |
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| 引用本文： | 赵伟春,程家安,张文俊.应用单克隆抗体评价拟环纹豹蛛对褐飞虱的控制作用[J].生态学报,2005,25(1):78-82. |
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| 作者姓名： | 赵伟春 程家安 张文俊 |
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| 作者单位： | 1. 浙江大学应用昆虫学研究所,杭州,310029;浙江中医学院生物工程学系,杭州,310053
2. 浙江大学应用昆虫学研究所,杭州,310029 |
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| 基金项目： | 国家“973”资助项目 ( G2 0 0 0 0 162 10 )，Rice IPM Network资助项目~~ |
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| 摘 要： | 应用褐飞虱的单克隆抗体 4 B8研究了 1999年浙江大学华家池校区农场汕优 6 3单季晚稻田中拟环纹豹蛛对褐飞虱的捕食作用。采用盘拍法的调查结果表明 ,褐飞虱、拟环纹豹蛛种群数量高峰期均在水稻生长后期 (9月中旬 ) ,最大种群密度分别为12 6头 /丛和 1.83头 /丛。对每次捕获的每头拟环纹豹蛛样品的抗体夹心 EL ISA检测结果表明 ,拟环纹豹蛛对褐飞虱单克隆抗体的阳性反应率与田间褐飞虱的生物量及拟环纹豹蛛对褐飞虱的占有量显著相关。定量评估结果表明 ,在此密度条件下 ,拟环纹豹蛛对褐飞虱的最大捕食率仅为 2 .2 8%。拟环纹豹蛛对褐飞虱的平均捕食量、总捕食量和捕食率与田间褐飞虱的生物量显著相关。总捕食量、捕食率与拟环纹豹蛛种群密度极显著相关
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| 关 键 词： | 褐飞虱 拟环纹豹蛛 单克隆抗体 酶联免疫吸附试验 捕食作用 |
| 文章编号： | 1000-0933(2005)01-0078-05 |
| 收稿时间： | 2003/10/14 0:00:00 |
| 修稿时间： | 2004/1/20 0:00:00 |
Evaluation of the control effects of Pardosa pseudoannulata on Nilaparvata lugens (st(a)l) with a monoclonal antibody |
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| ZHAO Weichun,CHEN Jia''an and ZHANG Wenjun.Evaluation of the control effects of Pardosa pseudoannulata on Nilaparvata lugens (st(a)l) with a monoclonal antibody[J].Acta Ecologica Sinica,2005,25(1):78-82. |
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| Authors: | ZHAO Weichun CHEN Jia'an and ZHANG Wenjun |
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| Institution: | Institute of Applied Entomology; Zhejiang University; Hangzhou; China |
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| Abstract: | Field surveys were carried out at five days interval in the single rice-growing season in Hangzhou in 1999. The rice crop were transplanted on June 23 and harvested on October 3. Using a plate inserted at the base of the plants, the numbers of Nilaparvata lugens and Pardosa pseudoannulata per hill were counted by eye. Counts were taken for 20 samples, 2 hills for each sample. Field investigation showed that the maximal densities of N. lugens and P. pseudoannulata were 126 /hill and 1.83 /hill, respectively.... |
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