不同地理种群羊草分子水平上生态型分化的研究

将来自内蒙古不同地区及吉林盐碱地的羊草（Ｌｅｙｍｕｓ ｃｈｉｎｅｎｓｉｓ）种子同时栽培于校园，以５个样点（阿旗定位站、西乌旗、谢尔塔拉、吉林）的羊草幼叶为材料，选用２０个随机短引物（１００ｎｔ）进行了ＤＮＡ随机扩增，共计获得１００个多态性片段。所得ＲＡＰＤ数据利用自编的计算机软件进行聚类分析、构建分子进行树，结果表明：定位站和西乌旗遗传距离最近，应为同一生态型；而吉林和谢尔塔拉分属不同生态型；阿旗

Comparative study on ecotype differentiation of Leymus chinensis in different geographic populations at moleculat level

The Leymus chinensis from different geographic populations were cultured in the campus and were used as materials in this research.Random amplified polymorphic DNA(RAPD) analysis was applied to these populations.Twenty random 10 mer primers yielded one hundred RAPD polymophic bands.Bands were scored ( /-) and un weighed pair group method with arithmetic mean(UPGMA) was used to generate dendrograms showing genetic relationships among the evaluated L.chinensis populations.Cluster analysis showed that Xiwuqi and Dingweizhan's populations have the smallest genetic distance and belong to the same ecotype;Xeltala and Jilin's may belong to the different ecotypes;Aqi's may be thought as other ecotype,because it has special characteristics.The result also made us sure that water was the most important factor among all that influence the ecotype differentiation among different geographic populations of Leymus chinensis .