| 6种植物次生物质对斜纹夜蛾解毒酶活性的影响 |
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| 引用本文： | 王瑞龙,孙玉林,梁笑婷,宋圆圆,苏贻娟,朱克岩,曾任森.6种植物次生物质对斜纹夜蛾解毒酶活性的影响[J].生态学报,2012,32(16):5191-5198. |
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| 作者姓名： | 王瑞龙 孙玉林 梁笑婷 宋圆圆 苏贻娟 朱克岩 曾任森 |
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| 作者单位： | 1. 华南农业大学亚热带农业生物资源保护与利用国家重点实验室,广州510642;农业部华南热带农业环境重点实验室,广州510642;广东省普通高等学校农业生态与农村环境重点实验室,广州510642
2. 德克萨斯农工大学昆虫学系,德克萨斯州77843美国 |
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| 基金项目： | 国家自然科学基金项目(31000260, 31100286, 31028018); 广东省自然科学基金项目(S2011040004336) |
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| 摘 要： | 草食性昆虫取食植物时遇到宿主植物中大量次生物质的化学防御,研究昆虫适应植物毒素的反防御策略具有重要的科学意义。分别添加0.01%肉桂酸、0.01%水杨酸、0.01%花椒毒素、0.02%槲皮素、0.05%黄酮和0.1%香豆素等6种植物次生物质的人工饲料饲养斜纹夜蛾(Spodoptera litura)五龄幼虫48 h后,测定斜纹夜蛾幼虫中肠和脂肪体中谷胱甘肽S-转移酶(GSTs)、羧酸酯酶(CarE)、P450的酶含量及头部乙酰胆碱酯酶(AChE)的活性,利用半定量RT-PCR检测中肠和脂肪体中CYP4M14和CYP4S9的基因表达水平。结果表明:取食肉桂酸和香豆素后,斜纹夜蛾中肠中CarE的酶活性分别提高了1.67和1.37倍,取食6种次生物质均能显著提高斜纹夜蛾脂肪体中GSTs酶活性。取食肉桂酸和香豆素48 h后,脂肪体中P450酶含量比对照增加2.93和14.50倍。取食肉桂酸、花椒毒素、槲皮素和香豆素后,斜纹夜蛾头部AchE酶活性与对照相比提高了1.53、1.80、2.36和1.56倍。6种次生物质均可诱导脂肪体中CYP4M14基因表达,槲皮素、肉桂酸和香豆素强烈诱导CYP4S9在脂肪体中表达。表明,斜纹夜蛾具有利用植物次生物质诱导其解毒酶的能力,进而提高其对毒素的抗性。
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| 关 键 词： | 斜纹夜蛾 解毒酶 细胞色素P450 植物次生物质 昆虫反防御策略 |
| 收稿时间： | 3/6/2012 12:00:00 AM |
| 修稿时间： | 2012/5/31 0:00:00 |
Effects of six plant secondary metabolites on activities of detoxification enzymes in Spodoptera litura |
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| WANG Ruilong,SUN Yulin,LIANG Xiaoting,SONG Yuanyuan,SU Yijuan,SALZMAN Keyan and ZENG Rensen.Effects of six plant secondary metabolites on activities of detoxification enzymes in Spodoptera litura[J].Acta Ecologica Sinica,2012,32(16):5191-5198. |
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| Authors: | WANG Ruilong SUN Yulin LIANG Xiaoting SONG Yuanyuan SU Yijuan SALZMAN Keyan and ZENG Rensen |
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| Institution: | State Key Laboratory for Conservation and Utilization of Subtropical Agro- bioresources, South China Agricultural University, Guangzhou 510642, China;Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China;Key Laboratory of Agroecology and Rural Environment of Guangdong Regular Higher Education Institutions, South China Agricultural University, Guangzhou 510642, China;State Key Laboratory for Conservation and Utilization of Subtropical Agro- bioresources, South China Agricultural University, Guangzhou 510642, China;Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China;Key Laboratory of Agroecology and Rural Environment of Guangdong Regular Higher Education Institutions, South China Agricultural University, Guangzhou 510642, China;State Key Laboratory for Conservation and Utilization of Subtropical Agro- bioresources, South China Agricultural University, Guangzhou 510642, China;Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China;Key Laboratory of Agroecology and Rural Environment of Guangdong Regular Higher Education Institutions, South China Agricultural University, Guangzhou 510642, China;State Key Laboratory for Conservation and Utilization of Subtropical Agro- bioresources, South China Agricultural University, Guangzhou 510642, China;Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China;Key Laboratory of Agroecology and Rural Environment of Guangdong Regular Higher Education Institutions, South China Agricultural University, Guangzhou 510642, China;State Key Laboratory for Conservation and Utilization of Subtropical Agro- bioresources, South China Agricultural University, Guangzhou 510642, China;Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China;Key Laboratory of Agroecology and Rural Environment of Guangdong Regular Higher Education Institutions, South China Agricultural University, Guangzhou 510642, China;Department of Entomology, Texas A&M University, College Station, Texas 77843, USA;State Key Laboratory for Conservation and Utilization of Subtropical Agro- bioresources, South China Agricultural University, Guangzhou 510642, China;Key Laboratory of Tropical Agro-environment, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China;Key Laboratory of Agroecology and Rural Environment of Guangdong Regular Higher Education Institutions, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | |
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| Keywords: | Spodoptera litura detoxification enzymes cytochrome P450 plant secondary metabolites insect counter-defense |
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