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| 低温应激对吉富罗非鱼血清生化指标及肝脏HSP70基因表达的影响 | |
| 引用本文： | 刘波,王美垚,谢骏,徐跑,戈贤平,何义进,缪凌鸿,潘良坤.低温应激对吉富罗非鱼血清生化指标及肝脏HSP70基因表达的影响[J].生态学报,2011,31(17):4866-4873. |
| 作者姓名： | 刘波 王美垚 谢骏 徐跑 戈贤平 何义进 缪凌鸿 潘良坤 |
| 作者单位： | 1. 南京农业大学(无锡)渔业学院,无锡214081;中国水产科学研究院淡水渔业研究中心,农业部淡水鱼类遗传育种和养殖生物学重点开放实验室,无锡214081 2. 南京农业大学(无锡)渔业学院,无锡,214081 3. 中国水产科学研究院淡水渔业研究中心,农业部淡水鱼类遗传育种和养殖生物学重点开放实验室,无锡214081 |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金项目(2011JBFA01);农业科技成果转化资金项目(2007GB2326037);农业部淡水鱼类遗传育种和养殖生物学重点开放实验室资助项目(BZ2007-01) |
| 摘 要： | 实验旨在探讨急性低温应激对吉富罗非鱼(GIFT,Oreochromis niloticus)血清生化、免疫指标以及肝脏HSP70 mRNA水平的影响。实验选取平均体重为(177±2.18)g左右的吉富罗非鱼作为实验对象,设定(25±1)℃对照组和低温(9±1)℃冷应激试验组,每组设定5个平行组,分别在0、2、6、12h随机采样,测定血清血糖(GLU)、胆固醇(CHOL) 、甘油三酯(TG)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、补体3 (C3)、补体4 (C4)、溶菌酶(LSZ)、皮质醇(COR)、三碘甲腺原氨酸(T3)、甲状腺素(T4)以及肝脏HSP70mRNA水平。结果表明:与对照组相比,试验组GLU水平在冷应激后6h,TG水平在冷应激后2、6h,CHOL水平在冷应激后6、12h均有显著升高(P<0.05),ALT水平在冷应激后2、12h均有显著升高(P<0.05);试验组AST、COR、T3水平在冷应激2、6、12h时出现显著升高(P<0.05),C3、C4、LSZ水平在冷应激后2、6、12h均出现显著下降(P<0.05);与对照组相比,试验组HSP70mRNA水平在冷应激后的12h出现显著升高(P<0.05)。因此,急性低温应激可以提高吉富罗非鱼肝脏应激蛋白HSP70mRNA水平,影响该鱼的非特异免疫力和相关生理指标。 |
| 关 键 词： | 吉富罗非鱼 低温应激 血液生理生化指标 HSP70 基因表达 |
| 收稿时间： | 7/5/2010 12:00:00 AM |
| 修稿时间： | 2011/2/14 0:00:00 |
Effects of acute cold stress onserum biochemical and immune parameters and liver HSP70 gene expression in GIFT strain of Nile tilapia (Oreochromis niloticus) |
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| LIU Bo,WANG Meiyao,XIE Jun,XU Pao,GE Xianping,HE Yijin,MIAO Linghong and PAN Liangkun.Effects of acute cold stress onserum biochemical and immune parameters and liver HSP70 gene expression in GIFT strain of Nile tilapia (Oreochromis niloticus)[J].Acta Ecologica Sinica,2011,31(17):4866-4873. | |
| Authors: | LIU Bo WANG Meiyao XIE Jun XU Pao GE Xianping HE Yijin MIAO Linghong and PAN Liangkun |
| Institution: | Wuxi Fishery College, Nanjing Agriculture University, Wuxi 214081,China;Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081,China;Wuxi Fishery College, Nanjing Agriculture University, Wuxi 214081,China;Wuxi Fishery College, Nanjing Agriculture University, Wuxi 214081,China;Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081,China;Wuxi Fishery College, Nanjing Agriculture University, Wuxi 214081,China;Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081,China;Wuxi Fishery College, Nanjing Agriculture University, Wuxi 214081,China;Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081,China;Wuxi Fishery College, Nanjing Agriculture University, Wuxi 214081,China;Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081,China;Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081,China;Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081,China |
| Abstract: | |
| Keywords: | GIFT cold stress serum biochemical parameters HSP70 mRNA |
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