| 棘胸蛙的性腺分化及温度对其性别决定的影响 |
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| 引用本文： | 梅祎芸,郑荣泉,郑善坚,严红,刘志芳,张启鹏,王志刚,洪燕.棘胸蛙的性腺分化及温度对其性别决定的影响[J].生态学报,2018,38(13):4809-4816. |
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| 作者姓名： | 梅祎芸 郑荣泉 郑善坚 严红 刘志芳 张启鹏 王志刚 洪燕 |
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| 作者单位： | 浙江师范大学野生动物保护与利用技术中心;浙江省野生动物生物技术与保护利用重点实验室;浙江师范大学行知学院 |
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| 基金项目： | 国家自然科学基金项目（31472015，31772482） |
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| 摘 要： | 单性养殖在棘胸蛙(Quasipaa spinosa)养殖中意义显著。为了了解棘胸蛙性腺分化,并探讨在不同的培育温度条件下性腺分化的差异。通过组织切片观察了棘胸蛙原始性腺的形成和性腺分化。棘胸蛙的性腺分化有其特殊性:生殖嵴形成时,其中既有体细胞,又有原始生殖细胞(PGCs);无论原始性腺是分化成为精巢还是卵巢,其中都出现一个带有单层扁平上皮初生性腔,当单层扁平上皮逐渐消失后形成次生性腔。性腔周围的PGCs开始长大2—3倍时,性腺将分化成为卵巢;体细胞渗入性腔中,使腔隙变小直至消失,这种原始性腺分化成为精巢。棘胸蛙蝌蚪孵化后的l7—80 d(Gosner 25—26期)为性腺分化的敏感时期。实验选取同一批次刚孵出蝌蚪(Gosner 24期),分别用不同水温(16±1)℃、(27±1)℃、(31±1)℃3组实验组及自然水温(23±1)℃对照组条件下的培育蝌蚪。结果显示,对照组的雌、雄性比为26∶24,雄性率接近50%;(16±1)℃实验组的雌、雄比例为33∶17,雄性率仅34%(P0.05);从(27±1)℃实验组起,雄性率提高,(31±1)℃实验组的雄性率达70%(P0.05)。棘胸蛙的性别分化属于温度依赖型性决定(TSD)。较高的培育温度可使棘胸蛙蝌蚪性别分化趋向雄性,而较低的培育温度则使蝌蚪雌性化。
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| 关 键 词： | 棘胸蛙 温度 性别决定 |
| 收稿时间： | 2017/6/27 0:00:00 |
| 修稿时间： | 2018/2/13 0:00:00 |
Gonad differentiation and the effects of temperature on sex determination in Quasipaa spinosa |
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| MEI Yiyun,ZHENG Rongquan,ZHENG Shanjian,YAN Hong,LIU Zhifang,ZHANG Qipeng,WANG Zhigang and HONG Yan.Gonad differentiation and the effects of temperature on sex determination in Quasipaa spinosa[J].Acta Ecologica Sinica,2018,38(13):4809-4816. |
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| Authors: | MEI Yiyun ZHENG Rongquan ZHENG Shanjian YAN Hong LIU Zhifang ZHANG Qipeng WANG Zhigang and HONG Yan |
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| Institution: | Center of Wildlife Conservation and Utilization Technology, Zhejiang Normal University, Jinhua 321004, China;Key Laboratory of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Jinhua 321004, China,Center of Wildlife Conservation and Utilization Technology, Zhejiang Normal University, Jinhua 321004, China;Key Laboratory of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Jinhua 321004, China;Xinzhi College, Zhejiang Normal University, Jinhua 321004, China,Center of Wildlife Conservation and Utilization Technology, Zhejiang Normal University, Jinhua 321004, China;Key Laboratory of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Jinhua 321004, China,Center of Wildlife Conservation and Utilization Technology, Zhejiang Normal University, Jinhua 321004, China;Key Laboratory of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Jinhua 321004, China,Center of Wildlife Conservation and Utilization Technology, Zhejiang Normal University, Jinhua 321004, China;Key Laboratory of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Jinhua 321004, China,Center of Wildlife Conservation and Utilization Technology, Zhejiang Normal University, Jinhua 321004, China;Key Laboratory of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Jinhua 321004, China,Center of Wildlife Conservation and Utilization Technology, Zhejiang Normal University, Jinhua 321004, China;Key Laboratory of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Jinhua 321004, China and Center of Wildlife Conservation and Utilization Technology, Zhejiang Normal University, Jinhua 321004, China;Key Laboratory of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Jinhua 321004, China |
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| Abstract: | |
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| Keywords: | Quasipaa spinosa temperature sex determination |
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