| 植物病原菌拮抗性野生艾蒿内生菌的分离、筛选和鉴定 |
| |
| 引用本文： | 徐亚军,赵龙飞,陈普,蒋晓瑞,韦革宏.植物病原菌拮抗性野生艾蒿内生菌的分离、筛选和鉴定[J].生态学报,2013,33(12):3697-3705. |
| |
| 作者姓名： | 徐亚军 赵龙飞 陈普 蒋晓瑞 韦革宏 |
| |
| 作者单位： | 1. 商丘师范学院生命科学学院,河南省高校植物与微生物互作重点实验室,河南省高校生物质降解与气化工程技术研究中心,商丘476000
2. 西北农林科技大学生命科学学院,旱区作物逆境生物学国家重点实验室,杨凌712100 |
| |
| 基金项目： | 国家自然科学基金(U1204301);河南省教育厅科学技术研究重点项目(12A210019);河南省高等学校青年骨干教师资助项目(2012GGJS166) |
| |
| 摘 要： | 从野生艾蒿的根茎叶部位分离出内生细菌共68株,以棉花枯萎病、稻瘟病、烟草赤星为供试病原菌,采用对峙法对内生菌分别进行抑菌试验,对筛选出的菌株进行了16S rDNA序列测定和系统发育分析。结果表明:从野生艾蒿分离到的内生菌经初筛、复筛,获得抑制病原菌效果最明显的3株菌,结合生理生化特性、菌落特征、细胞形态特征和16S rDNA测序分析结果,菌株L8、S11和R6分别鉴定为Bacillus subtilis,Bacillus cereus,Paenibacillus polymyxa。拮抗实验表明,棉花枯萎病原菌菌丝发生弯曲、打结,烟草赤星的受作用菌丝生长端分枝明显增多,生长端边缘呈珊瑚状分枝,并且出现明显的畸形和萎缩现象。分析表明,可能是由于在培养过程中内生菌产生了化感物质,对病原菌的菌丝产生抑制作用的结果。
|
| 关 键 词： | 野生艾蒿 内生细菌 植物病原菌 抑菌作用 芽孢杆菌属 |
| 收稿时间： | 2012/12/6 0:00:00 |
| 修稿时间： | 3/4/2013 12:00:00 AM |
Isolation, screening and characterization of phytopathogen antagonistic endophytes from wild Artemisia argyi |
| |
| XU Yajun,ZHAO Longfei,CHEN Pu,JIANG Xiaorui and WEI Gehong.Isolation, screening and characterization of phytopathogen antagonistic endophytes from wild Artemisia argyi[J].Acta Ecologica Sinica,2013,33(12):3697-3705. |
| |
| Authors: | XU Yajun ZHAO Longfei CHEN Pu JIANG Xiaorui and WEI Gehong |
| |
| Institution: | College of Life Sciences, Shangqiu Normal University, Key Laboratory of Plant-Microbe Interactions of Henan, Engineering Technology Research Center of Biomass Degradation and Gasification of Henan University, Henan Shangqiu 476000, China;College of Life Sciences, Shangqiu Normal University, Key Laboratory of Plant-Microbe Interactions of Henan, Engineering Technology Research Center of Biomass Degradation and Gasification of Henan University, Henan Shangqiu 476000, China;College of Life Sciences, Shangqiu Normal University, Key Laboratory of Plant-Microbe Interactions of Henan, Engineering Technology Research Center of Biomass Degradation and Gasification of Henan University, Henan Shangqiu 476000, China;College of Life Sciences, Shangqiu Normal University, Key Laboratory of Plant-Microbe Interactions of Henan, Engineering Technology Research Center of Biomass Degradation and Gasification of Henan University, Henan Shangqiu 476000, China;College of Life Sciences, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling, Shaanxi, 712100, China |
| |
| Abstract: | A total of 68 endophytes isolated from roots, stems and leaves of wild Artemisia argyi were used to study antagonistic effect against phytopathogens including Fusarium oxysporum, Magnaporthe grisea and Alternaria alternate, and characterized by 16S rDNA sequencing and phylogenies. The results indicated that three of the endophytes have the most obvious inhibitory effect by the first and the second screening. Combined with the physiological and biochemical characteristics, colony characteristics, cell morphological characteristics and 16S rDNA sequencing results, the three strains L8, S11 and R6 were identified as Bacillus subtilis, Bacillus cereus, Paenibacillus polymyxa, respectively. Antagonistic experiment showed that hyphae of Fusarium oxysporum became bending and knot while hyphae of Alternaria alternata grew more branches. The growth end appeared coralline branch with deformity and atrophy, suggesting that allelochemicals produced by endophytes in the development process, might be inhibit the development of pathogens hyphae. |
| |
| Keywords: | Artemisia argyi endophytic bacteria phytopathogen antifungal activity Bacillus |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《生态学报》浏览原始摘要信息 |
| 点击此处可从《生态学报》下载免费的PDF全文 |
|
