| 华北及西北地区岩黄芪根瘤菌的表型及遗传多样性 |
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| 引用本文： | 刘洋,隋新华,陈文新.华北及西北地区岩黄芪根瘤菌的表型及遗传多样性[J].生态学报,2005,25(5):1088-1094. |
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| 作者姓名： | 刘洋 隋新华 陈文新 |
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| 作者单位： | 中国农业大学生物学院,农业部农业微生物资源及其应用重点开放实验室,北京,100094 |
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| 基金项目： | 国家重点基础研究发展规划资助项目 (2 0 0 1CB10 890 5 )，国家高技术研究发展计划资助项目 (2 0 0 3 AA2 4115 0 )，国家自然科学基金资助项目 (3 0 2 70 0 0 1)~~ |
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| 摘 要： | 选用分离自河北、内蒙古等5省区的岩黄芪根瘤菌30株及17株已知参比菌株,进行了营养利用、抗生素抗性、耐逆性及生理生化反应等12 5项表型性状研究,所得数值分类树状图表明不同宿主及不同地域的岩黄芪根瘤菌的表型多样性。通过对其中部分菌株进行16 S r DNA PCR- RFL P及BOX- PCR指纹图谱分析,聚类结果表明供试岩黄芪根瘤菌具有遗传多样性。
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| 关 键 词： | 岩黄芪根瘤菌 多样性 数值分类 16S rDNA PCR-RFLP BOX-PCR指纹图谱 |
| 文章编号： | 1000-0933(2005)05-1088-07 |
| 收稿时间： | 2004/5/11 0:00:00 |
| 修稿时间： | 2004年5月11日 |
Phenotypic and genetic diversity of rhizobia isolated from root modules of Hedysarum spp. grown in the northern and northwestern regions of China |
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| LIU Yang,SUI Xinhua and CHEN Wenxin.Phenotypic and genetic diversity of rhizobia isolated from root modules of Hedysarum spp. grown in the northern and northwestern regions of China[J].Acta Ecologica Sinica,2005,25(5):1088-1094. |
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| Authors: | LIU Yang SUI Xinhua and CHEN Wenxin |
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| Institution: | College of Biological Sciences; China Agriculture University |
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| Abstract: | The legume species within the genus Hedysarum are deep-rooted annual or perennial herbs, and they are well distributed in the temperate zones. About 40 species of Hedysarum are native to China and they are potential foliages and are used as resources of herbal medicine. These legumes are pioneers plants, but its geographic distribution may be determined by the existence of proper rhizobia. Aiming at understanding the diversity of rhizobia associated with them, we isolated thirty rhizobial strains from root ... |
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| Keywords: | Hedysarum rhizobia diversity numerical taxonomy 16S rDNA PCR-RFLP BOX-PCR fingerprinting |
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