| 氮磷添加对内蒙古温带典型草原土壤微生物群落结构的影响 |
| |
| 引用本文： | 施瑶,王忠强,张心昱,孙晓敏,刘希玉,何念鹏,庾强.氮磷添加对内蒙古温带典型草原土壤微生物群落结构的影响[J].生态学报,2014,34(17):4943-4949. |
| |
| 作者姓名： | 施瑶 王忠强 张心昱 孙晓敏 刘希玉 何念鹏 庾强 |
| |
| 作者单位： | 东北师范大学地理科学学院, 长春 130024;中国科学院地理科学与资源研究所生态系统网络观测与模拟重点实验室, 北京 100101;东北师范大学地理科学学院, 长春 130024;中国科学院地理科学与资源研究所生态系统网络观测与模拟重点实验室, 北京 100101;中国科学院地理科学与资源研究所生态系统网络观测与模拟重点实验室, 北京 100101;东北师范大学地理科学学院, 长春 130024;中国科学院地理科学与资源研究所生态系统网络观测与模拟重点实验室, 北京 100101;中国科学院地理科学与资源研究所生态系统网络观测与模拟重点实验室, 北京 100101;中国科学院沈阳应用生态研究所, 沈阳 110016 |
| |
| 基金项目： | 国家自然科学基金面上项目（41171153）；中国科学院知识创新工程重要方向项目（KZCX2-EW-310） |
| |
| 摘 要： | 选取内蒙古温带典型草原,进行连续6a氮磷添加试验,采用土壤特征微生物PLFA生物标记技术,研究6个氮添加水平N0(0 kg N hm-2a-1)、N1(56 kg N hm-2a-1)、N2(112 kg N hm-2a-1)、N3(224 kg N hm-2a-1)、N4(392 kg N hm-2a-1)、N5(560 kg N hm-2a-1)和6个磷添加水平P0(0 kg P hm-2a-1)、P1(15.5 kg P hm-2a-1)、P2(31 kg P hm-2a-1)、P3(62 kg P hm-2a-1)、P4(93 kg P hm-2a-1)、P5(124 kg P hm-2a-1)对土壤特征微生物PLFA生物标记数量和土壤微生物群落结构的影响。结果表明:(1)随氮添加量增加,土壤微生物总磷脂脂肪酸(PLFA)含量和土壤细菌PLFA生物标记数量、放线菌PLFA生物标记数量呈上升趋势,土壤G+/G-呈增加趋势;各氮添加水平对土壤真菌PLFA生物标记数量无显著差异,随氮添加量增加,土壤真菌/细菌比降低。(2)随磷添加量增加,土壤总磷脂脂肪酸(PLFA)含量、土壤细菌PLFA生物标记数量、放线菌PLFA生物标记数量、真菌PLFA生物标记数量及真菌/细菌比值呈先上升后下降趋势,均以P3水平(62 kg P hm-2a-1)处理最高,说明适宜的磷添加对内蒙古温带典型草原土壤微生物繁殖和菌落结构有显著影响。
|
| 关 键 词： | 温带典型草原 氮磷添加 磷脂脂肪酸（PLFA） 细菌 真菌 放线菌 微生物群落结构 |
| 收稿时间： | 6/8/2013 12:00:00 AM |
| 修稿时间： | 2014/6/27 0:00:00 |
Effects of nitrogen and phosphorus addition on soil microbial community composition in temperate typical grassland in Inner Mongolia |
| |
| SHI Yao,WANG Zhongqiang,ZHANG Xinyu,SUN Xiaomin,LIU Xiyu,HE Nianpeng and YU Qiang.Effects of nitrogen and phosphorus addition on soil microbial community composition in temperate typical grassland in Inner Mongolia[J].Acta Ecologica Sinica,2014,34(17):4943-4949. |
| |
| Authors: | SHI Yao WANG Zhongqiang ZHANG Xinyu SUN Xiaomin LIU Xiyu HE Nianpeng and YU Qiang |
| |
| Institution: | School of Geographical Science, Northeast Normal University, Changchun 130024, China;Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;School of Geographical Science, Northeast Normal University, Changchun 130024, China;Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;School of Geographical Science, Northeast Normal University, Changchun 130024, China;Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China |
| |
| Abstract: | Nitrogen and phosphorus addition is a main measure to improve plant growth and soil properties. However, little is known about the relationship between nitrogen and phosphorus addition and soil microbial community composition in temperate typical grassland in Inner Mongolia. In this paper, two single factor experiments were conducted respectively to investigate the influence of different nitrogen rates (0, 56, 112, 224, 392, 560 kg N hm-2 a-1) and different phosphorus rates (0, 15.5, 31, 62, 93, 124 kg P hm-2 a-1) addition on soil microbial community composition of temperate typical grassland in Xilinguole, Inner Mongolia, China. During the 6-year experimental period, increasing nitrogen rate increased the total numerical values of PLFA biomarker, actinomycete PLFA biomarker, bacteria PLFA biomarker, and the ratios of G+/G-, but did not have significant effect on the numerical values of fungal PLFA biomarker. Increasing nitrogen rate decreased the ratios of fungi/bacteria. Total numerical values of PLFA biomarker, bacteria PLFA biomarker, actinomycete PLFA biomarker, fungi PLFA biomarker and fungi PLFA biomarker/bacterial PLFA biomarker ratio had the highest values at the P3 (62 kg P hm-2 a-1)phosphorus addition treatment. The results indicated that nitrogen and phosphorus addition could greatly affect soil microbial community composition in temperate typical grassland. |
| |
| Keywords: | temperate typical grassland nitrogen and phosphorus addition PLFA biomarker bacteria fungi actinomycete soil microbial community |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《生态学报》浏览原始摘要信息 |
| 点击此处可从《生态学报》下载免费的PDF全文 |
|
