Ni~(2+)对不同发育阶段斜纹夜蛾虫体血淋巴能量物质的影响

研究了饲料中不同浓度的Ni2+(140mg/kg)对连续3个世代斜纹夜蛾Spodoptera litura Fabricius6龄末幼虫、蛹和成虫血淋巴中能量物质总糖、蛋白质和脂肪含量及血淋巴中热量值的影响。结果表明,Ni2+胁迫1个世代,6龄末幼虫、蛹和成虫血淋巴中的总糖含量均低于对照,而血淋巴中的蛋白质含量则在低浓度Ni2+胁迫下增高,在高浓度Ni2+胁迫下降低;只有受120mg/kg Ni2+胁迫的6龄末幼虫血淋巴的脂肪含量高于对照,而蛹和成虫血淋巴中的脂肪含量及热量值均低于对照。在Ni2+胁迫的第2、3代,6龄末幼虫、蛹和成虫血淋巴中的总糖、蛋白含量和热量值与饲料中Ni2+浓度的反应关系均表现为低浓度Ni2+胁迫增加而高浓度Ni2+胁迫降低的趋势;然而,血淋巴中的脂肪含量随饲料中Ni2+浓度的增加而降低。因此,重金属Ni2+对S.litura能量物质的影响与虫体内能量物质的种类和虫体的发育阶段有一定的关联。

Effects of dietary nickel on the energy reserves in the hemolymph of different developmental stages of Spodoptera litura Fabricius

Effects of dietary nickel on total sugars, protein and total lipids in the hemolymph of the 6th instar larvae, pupae and adults of Spodoptera litura Fabricius were investigateed by exposing S. litura larvae to the artificial diets amended with different concentrations of nickel for 3 generations. It was indicated that 1 generation exposure lowered the total sugars in the hemolymph of the 6th instar larvae, pupae and audlts in comparison with those in control. Proteins in the hemolymph of different developmental stages of S. litura increased at low levels of nickel, but decreased at high levles of nickel. Higher concentrations of total lipids were only occurred in the 6th instar larvae of the 1st generation exposed to 1-20 mg/kg nickel comparing to that in control. Total lipids and calorie in the pupae and adults were lower than those in control in the 1st generation. Dose-dependent responses of total sugars, protein and calorie in hemolymph of the 6th instar larvae, pupae and adults in the 2nd and 3rd generations were characterized as stimulation at low levels of nickel, and inhibition at high levels of nickel. However, total lipids in hemolymph of the 6th instar larvae, pupae and adults in the 2nd and 3rd generations were all lower than those in control. Hence, effects of dietary nickel on the energy substances in the hemolymph of S. litura were correlated with the type of energy materials and the developmental stages of S. litura.