| 木论喀斯特常绿落叶阔叶混交林土壤细菌多样性及其最优采样数 |
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| 引用本文： | 陈莉,宋同清,王华,曾馥平,彭晚霞,杜虎,苏樑.木论喀斯特常绿落叶阔叶混交林土壤细菌多样性及其最优采样数[J].生态学报,2019,39(9):3287-3296. |
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| 作者姓名： | 陈莉 宋同清 王华 曾馥平 彭晚霞 杜虎 苏樑 |
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| 作者单位： | 中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室;湖南农业大学生物科学技术学院;中国科学院环江喀斯特生态系统观测研究站 |
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| 基金项目： | 国家重点研发计划项目（2016YFC0502405）；广西重点研发计划项目（桂科AB16380255，桂科AB17129009）；国家自然科学基金项目（31770495，31660141） |
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| 摘 要： | 土壤微生物在森林生态系统中起着重要作用。为了了解木论喀斯特常绿落叶阔叶混交林土壤细菌的多样性,明确采样数对多样性的影响,采用Illumina Hiseq高通量测序技术对木论喀斯特常绿落叶阔叶混交林土壤细菌多样性进行了初步研究,发现:在82个采样点内,随着采样点增加,不同分类水平的土壤细菌类群数量有增多趋势;当采样点达到82个时,检测出的土壤细菌类群达44门99纲180目356科630属和269822个OTU;82个土壤样品中所检测出的细菌类群平均有28.8门60.9纲108.1目206.8科239.6属和3290.5个OTU,其中门、纲、目分类水平上优势类群所占比例分别为变形菌门(Proteobacteria)(34.51%)、α-变形菌纲(Alphaproteobacteria)(18.43%)、根瘤菌目(Rhizobiales)(12.50%)。取样数量对检测出的土壤细菌类群数量有显著影响:在门、纲、目、科的分类水平上,36个、57个、72个、78个样品基本上可代表82个样品监测出来不同分类水平的细菌多样性信息;在属和OTU的分类水平上,当样品数量达到82个时检测出来的相应细菌类群数仍在不断地增加。这些初步的研究结果显示木论常绿落叶阔叶混交林土壤在一定程度上有着较高的细菌多样性水平,该研究为细菌多样性与植物多样性的进一步研究奠定了基础。
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| 关 键 词： | 喀斯特 细菌多样性 土壤微生物 森林 高通量测序 |
| 收稿时间： | 2017/11/15 0:00:00 |
| 修稿时间： | 2018/11/21 0:00:00 |
Soil bacterial diversity and optimal sampling number in the Mulun karst evergreen and deciduous broad-leaved mixed forest |
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| CHEN Li,SONG Tongqing,WANG Hu,ZENG Fuping,PENG Wanxi,DU Hu and SU Liang.Soil bacterial diversity and optimal sampling number in the Mulun karst evergreen and deciduous broad-leaved mixed forest[J].Acta Ecologica Sinica,2019,39(9):3287-3296. |
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| Authors: | CHEN Li SONG Tongqing WANG Hu ZENG Fuping PENG Wanxi DU Hu and SU Liang |
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| Institution: | Key laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China;Huanjiang Observation and Research Station of Karst Ecosystem, Chinese Academy of Sciences, Huanjiang 547100, China,Key laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;Huanjiang Observation and Research Station of Karst Ecosystem, Chinese Academy of Sciences, Huanjiang 547100, China,Key laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China,Key laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;Huanjiang Observation and Research Station of Karst Ecosystem, Chinese Academy of Sciences, Huanjiang 547100, China,Key laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;Huanjiang Observation and Research Station of Karst Ecosystem, Chinese Academy of Sciences, Huanjiang 547100, China,Key laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;Huanjiang Observation and Research Station of Karst Ecosystem, Chinese Academy of Sciences, Huanjiang 547100, China and Key laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China;Huanjiang Observation and Research Station of Karst Ecosystem, Chinese Academy of Sciences, Huanjiang 547100, China |
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| Abstract: | |
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| Keywords: | Karst bacterial diversity soil microbe forest high-throughput sequencing |
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