外生菌根菌对油松幼苗抗氧化酶活性及根系构型的影响

研究盆栽条件下,接种褐环乳牛肝菌(Suillus luteus)和红汁乳菇(Lactarius hatsudake)对油松(Pinus tabulaeformis)苗木生长,抗氧化酶活性和根系构型的影响。结果表明:(1)褐环乳牛肝菌和红汁乳菇均可与油松合成外生菌根,并显著提高苗木的苗高、地径、鲜重和干重。(2)接种褐环乳牛肝菌苗木针叶抗氧化酶(SOD、CAT、POD)活性分别比接种红汁乳菇苗木高14.77%、20.77%、34.68%;接种褐环乳牛肝菌苗木根系抗氧化酶(SOD、CAT、POD)活性分别比接种红汁乳菇苗木高8.54%,4.34%,33.31%;接种处理苗木抗氧化酶活性均显著高于不接种处理。接种处理苗木根长、表面积、体积、平均直径、根尖数、分叉数显著高于不接种处理;接种处理间苗木只有根尖数存在显著差异;接种褐环乳牛肝菌的油松根系分支成80°—90°的一级侧根数占23.81%,显著多于接种红汁乳菇和对照苗木。研究表明,接种褐环乳牛肝菌和红汁乳菇均能促进油松苗木生长,提高油松体内抗氧化酶活性,扩大苗木根系的吸收范围,其中褐环乳牛肝菌各方面的促进效果要优于红汁乳菇。

Effects of inoculation of exogenous mycorrhizal fungi on the antioxidant and root configuration enzyme activity of Pinus tabulaeformis seedlings

The seedlings of Pinus tabulaeformis were inoculated with Lactarius hatsudake and Suillus luteus under pot culture conditions, and the growth characteristics, anti-oxidase activity and root architecture of seedlings after the formation of ectomycorrhizal fungi were examined. The results showed that: (1) The two exogenous mycorrhizal fungi variables can form exogenous mycorrhizae with P. tabulaeformis and the seedling height, ground diameter, fresh weight, and dry weight were significantly improved; (2) In the needles treated with S. luteus, the activity of superoxide dismutase (SOD) was 14.77% higher than that after L. hatsudake treatment, peroxidase (POD) was 20.77%, and catalase (CAT) was 34.68%. In roots treated with S. luteus, the activities of SOD, POD, and CAT were 8.54%, 4.34%, and 33.31% higher than those after L. hatsudake treatment, respectively. All root indices (root length, surface area, volume, average root diameter, number of root tips, and branching) and anti-oxidase activity with the two treatments were improved significantly. Among all root indices, there was only a significant difference in the number of root tips between the inoculation of L. hatsudake and S. luteus. Following S. luteus treatment, the seedling primary lateral roots number in the 80°-90° angle of branch was 23.81%, which was significantly greater than that following L. hatsudake treatment and in the control group (CK). Based on these results, S. luteus and L. hatsudake treatments improved seedling growth, enhanced the anti-oxidase activity of the seedlings, and enlarged the absorption range of the roots. Compare to L. hatsudake treatments, S. luteus treatment has better promotion effects on all seedling indices.