构树(Broussonetia papyrifera)幼苗氮、磷吸收对接种AM真菌的响应

对石灰岩适生植物构树(Broussonetia papyrifera)进行菌根真菌摩西球囊霉(Glomus mosseea)、地表球囊霉(G.versiforme)、透光球囊霉(G.diaphanum)的单独接种、混合接种和不接种处理，幼苗生长3个月后测定其根茎叶N、P含量和土壤酶活性，结果表明：接种AM真菌后构树幼苗生物量显著增加，植株根茎叶N含量较对照组显著提高，其含量为根<茎<叶。各处理中除了透光球囊霉处理的茎含量与对照差异不显著外,其余各处理含量均呈显著或极显著差异。AM真菌对构树苗P的促进效应主要体现在根、茎的利用上，而叶片P含量除混合接种外则有所降低，但M+(接种处理)与M-(非接种处理)间无显著差异。构树苗根茎叶等量干物质P含量依次为叶>根>茎。进一步研究表明，接种AM真菌显著提高了土壤酶活性，这种显著性主要体现在蛋白酶和碱性磷酸酶上, 植株N含量与土壤蛋白酶和碱性磷酸酶活性有显著相关性，而P含量只是根部吸收与多酚氧化酶活性显著相关。结果表明，接种AM真菌促进了宿主植物构树生物量的积累，提高了根际土壤酶活性，增加了植株对N、P的吸收。

Response of N and P absorption on Broussonetia papyrifera seedlings to inoculate Vesicular-arbuscular mycorrhizal fungus

An experiment was conducted that Glomus mosseea, Glomus versiforme and Glomus.diaphanum were inoculated to Broussonetia papyrifera seedlings with single inoculation, co-inoculation and non-inoculation. Three months later, concentrations of nitrogen and phosphorus and soil enzyme activities seedlings were measured. The results showed that nitrogen concentration of host plants was increased significantly in root, stem and leaf comparing with none-inoculated, the order was rootstem<leaf. Activities of four kinds of soil enzymes (Polyphenol oxidase, Protease, Alkaline phosphatase, Peroxide hydrogenase) increased, and activities of Protease and Alkaline phosphatase were significant difference between inoculation and none disposals. A significant correlation was found between nitrogen concentration with Protease and Alkaline phosphatase, phosphorus concentration was correlated with Polyphenol oxidase. We conclude treatments of inoculation on AM fungus increase contents of N and P host plant with biomass and soil enzyme activites.