环境微生物样品真菌群落BIOLOG分析方法

利用BIOLOG YT、FF微孔板分别考察了4个真菌群落代谢活性及群落间的代谢相似性,并与聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)结构相似性分析对比试图探讨代谢相似性与结构相似性的内在联系,探讨了超低温冻存法作为样品保存手段对真菌群落特征BIOLOG分析结果的影响.结果表明:两种微孔板所反映的代谢相似性聚类分析结果完全不同, FF板所反映的代谢相似性聚类分析规律与PCR-DGGE提供的种群结构聚类分析规律一致;超低温冻存处理影响显著影响BIOLOG YT代谢活性(P = 0.023)和BIOLOG FF多样性指数(H')(P = 0.041),但对两种微孔板所反映的其它指数如代谢活性、丰富度指数(S)、多样性指数(H')分析结果均无显著性影响(P > 0.05).

BIOLOG analysis for fungal communities in environmental samples

BIOLOG YT and FF microplates were used in this study to investigate the metabolic activities and similarity of 4 fungal communities in environmental samples. Subsequently, the BIOLOG metabolic similarity expressed by these two microplates was linked to structural similarity expressed by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE). In addition, the effect of cryopreservation on BIOLOG analytic results, including metabolic activities, richness index and diversity index, was also discussed. The following conclusions have been drawn: (1) BIOLOG FF microplate expressed a metabolic similarity relation among 4 fungal communities absolutely different from that by BIOLOG YT microplate. (2) The BIOLOG FF metabolic similarity of 4 fungal communities nearly corresponded to the PCR-DGGE structural similarity. (3) The cryopreservation had no significant influence on BIOLOG richness index, BIOLOG FF metabolic activities and BIOOG YT diversity index (P > 0.05).