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太湖水华期间有毒和无毒微囊藻种群丰度的动态变化
引用本文:李大命,叶琳琳,于洋,张民,阳振,孔繁翔.太湖水华期间有毒和无毒微囊藻种群丰度的动态变化[J].生态学报,2012,32(22):7109-7116.
作者姓名:李大命  叶琳琳  于洋  张民  阳振  孔繁翔
作者单位:江苏省淡水水产研究所, 南京 210097;中国科学院南京地理与湖泊研究所,湖泊与环境国家重点实验室, 南京 210008;中国科学院研究生院, 北京 100049;中国科学院南京地理与湖泊研究所,湖泊与环境国家重点实验室, 南京 210008;中国科学院研究生院, 北京 100049;中国科学院南京地理与湖泊研究所,湖泊与环境国家重点实验室, 南京 210008;中国科学院南京地理与湖泊研究所,湖泊与环境国家重点实验室, 南京 210008;中国科学院南京地理与湖泊研究所,湖泊与环境国家重点实验室, 南京 210008;中国科学院南京地理与湖泊研究所,湖泊与环境国家重点实验室, 南京 210008
基金项目:973计划项目(2008CB418000);中国科学院南京地理与湖泊所所长基金(07SL021001)
摘    要:采用荧光定量PCR技术分析太湖3个湖区(梅梁湾、贡湖湾和湖心)水体中有毒和无毒微囊藻基因型丰度及有毒微囊藻比例的季节变化(2010年4-9月),并与环境因子进行统计分析。结果表明,有毒微囊藻基因型丰度及所占比例存在季节和空间差异:从4-8月,有毒微囊藻基因型丰度及其比例呈逐渐增加趋势,到9月开始下降;梅梁湾水体中有毒微囊藻基因型丰度及其比例高于贡湖湾和湖心。梅梁湾、贡湖湾和湖心有毒微囊藻在微囊藻种群中的比例变化范围分别为(26.2±0.8)%-(64.3±2.2)%、(4.4±0.2)%-(22.1±1.8)%和(10.4±0.4)%-(20.6±1.5)%。相关分析结果表明,有毒微囊藻丰度、总微囊藻丰度和叶绿素a浓度呈极显著正相关(P<0.01),均与温度呈显著正相关(P<0.05);有毒微囊藻比例与磷浓度呈显著正相关(P<0.05),与温度呈极显著正相关(P<0.01)。研究结果表明,温度和磷浓度是决定太湖有毒微囊藻种群丰度及其比例的关键因子。

关 键 词:蓝藻水华  有毒微囊藻  荧光定量PCR  太湖
收稿时间:2011/10/17 0:00:00
修稿时间:2012/5/29 0:00:00

Dynamics of toxic and non-toxic Microcystis spp. during bloom in the large shallow hypereutrophic Lake Taihu
LI Daming,YE Linlin,YU Yang,ZHANG Min,YANG Zhen and KONG Fanxiang.Dynamics of toxic and non-toxic Microcystis spp. during bloom in the large shallow hypereutrophic Lake Taihu[J].Acta Ecologica Sinica,2012,32(22):7109-7116.
Authors:LI Daming  YE Linlin  YU Yang  ZHANG Min  YANG Zhen and KONG Fanxiang
Institution:Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210097, China;State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China;Graduate University of Chinese Academy of Sciences, Beijing 100049, China;State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China;Graduate University of Chinese Academy of Sciences, Beijing 100049, China;State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China;State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China;State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China;State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China
Abstract:Lake Taihu is a large(2338 km2), shallow(mean depth 2.0 m), hypereutrophic and well mixed year-round(polymictic) lake. For the past three decades, seasonal blooms of Microcystis spp. have regularly formed in the lake, usually starting around April and persisting through October. It has long been known that microcystin-producing and non microcystin-producing strains can be isolated from one water sample, and the waxing and waning of microcystin- producing versus non-microcystin-producing strains has been suggested as a most important factor regulating net microcystin production in water. However, the quantification of microcystin producers versus non-microcystin-producers was for a long time impeded because of the morphological similarity between strains differing in microcystin production. Quantitative real-time PCR(qPCR) has been successfully applied to track quantities of microcystin-producing Microcystis genotypes in natural populations. So far, the effects of environmental factors on the abundance of microcystin-producing and non-toxic Microcystis genotypes have been studied in a relatively limited scale. In this study, We attempted to verify the environmental factors related to the spatial and temporal dynamics of the abundance of toxic and non-toxic Microcystis in the Lake Taihu. Water samples were collected in three sampling sites(Meiliang Bay(N2), Gonghu Bay(N4) and the center area(S4) at monthly intervals from April to September, 2010. The abundance and subpopulation proportion of toxic Microcystis genotypes was quantified using a quantitative real-time PCR with two primers sets for the cpcBA-IGS and mcyA gene. QPCR data showed that the abundance and proportion of toxic Microcystis genotypes in three studying sites increased gradually from April to August, and then decreased in September, with both values being higher in Meiliang Bay than in the other two sites. The proportion of toxic Microcystis genotypes in Meiliang, GonghuBay and the center area varied considerably, ranging from (26.2± 0.8)% to (64.3 ±2.2)%, from (4.4±0.2)% to (22.1±1.8)% and from (10.4±0.4)% to (20.6±1.5)%, respectively. Correlation analysis revealed a significantly positive relationship between chlorophyll a, toxic Microcystis and total Microcystis. Meanwhile all the three components were strongly related to water temperature(P<0.05). The change of the proportion of toxic Microcystis genotypes was closed related to water temperature(P<0.01) and phosphorus concentration(P<0.05), suggesting that water temperature and phosphorus concentration are critical environmental factors for determining the abundance and proportion of toxic Microcystis genotypes in Lake Taihu.
Keywords:cyanobacterial bloom  toxic Microcystis  quantitative real-time PCR  Lake Taihu
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