| 氮磷添加对杉木林土壤碳氮矿化速率及酶动力学特征的影响 |
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| 引用本文： | 聂二旗,张心昱,郑国砥,杨洋,王辉民,陈伏生,孙晓敏.氮磷添加对杉木林土壤碳氮矿化速率及酶动力学特征的影响[J].生态学报,2018,38(2):615-623. |
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| 作者姓名： | 聂二旗 张心昱 郑国砥 杨洋 王辉民 陈伏生 孙晓敏 |
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| 作者单位： | 中国科学院地理科学与资源研究所生态系统网络观测与模拟重点实验室;中国科学院地理科学与资源研究所环境修复中心;中国科学院大学;江西农业大学江西省竹子种质资源与利用重点实验室; |
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| 基金项目： | 国家自然科学基金项目（41571251，41571130043）；中科院技术创新项目（201604） |
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| 摘 要： | 以江西杉木林红壤为研究对象,开展野外长期氮(N)、磷(P)添加控制试验,设置对照(CK)、N(50kg N hm~(-2)a~(-1))、P(50kg P hm~(-2)a~(-1))、NP(50kg N hm~(-2)a~(-1)+50kg P hm~(-2)a~(-1))处理,分析N、P添加对土壤碳矿化速率(C_(min))、氮矿化速率(N_(min))和相关的β-1,4-葡萄糖苷酶(βG)和β-1,4-N-乙酰葡糖氨糖苷酶(NAG)动力学参数的影响。结果表明:(1)N添加明显降低了C_(min)和N_(min),比CK分别减少了25%和18%;N添加减小了NAG的潜在最大酶活性(V_(max))、半饱和常数(K_m)、催化效率(V_(max)/K_m),但差异不显著(P0.05);N添加显著增加了βG的V_(max)、K_m,但对V_(max)/K_m有抑制作用。(2)P输入(P、NP)较CK使NAG的V_(max)、K_m减小26%—60%;NP同时添加明显提高βG和NAG的V_(max)/K_m(P0.05),但P输入(P、NP)对C_(min)和N_(min)影响不显著(P0.05)。(3)C_(min)与土壤溶解性有机碳正相关,N_(min)与pH显著正相关,与土壤NH_4~+-N、NO_3~--N显著负相关;βG和NAG的V_(max)/K_m均与NH_4~+-N、NO_3~--N负相关(P0.05),K_m均与NH_4~+-N、NO_3~--N正相关(P0.05)。βG的V_(max)与NH_4~+-N、NO_3~--N正相关(P0.05),NAG的V_(max)与有机碳、全氮、全磷、有效磷负相关(P0.05)。研究结果表明,在亚热带杉木人工林中,N添加降低土壤pH,增加土壤有效氮含量,抑制βG和NAG的V_(max)/K_m,对土壤C_(min)和N_(min)产生抑制作用;而NP添加增加土壤有效磷含量,增加土壤βG和NAG的V_(max)/K_m。
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| 关 键 词： | 氮磷添加 碳氮矿化 酶动力学 水解酶 亚热带红壤 |
| 收稿时间： | 2016/8/23 0:00:00 |
Effects of nitrogen and phosphorus additions on soil organic carbon and nitrogen mineralization and hydrolase kinetics in Chinese fir plantations |
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| NIE Erqi,ZHANG Xinyu,ZHENG Guodi,YANG Yang,WANG Huimin,CHEN Fusheng and SUN Xiaomin.Effects of nitrogen and phosphorus additions on soil organic carbon and nitrogen mineralization and hydrolase kinetics in Chinese fir plantations[J].Acta Ecologica Sinica,2018,38(2):615-623. |
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| Authors: | NIE Erqi ZHANG Xinyu ZHENG Guodi YANG Yang WANG Huimin CHEN Fusheng and SUN Xiaomin |
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| Institution: | Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;Center for Environmental Remediation, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;University of Chinese Academy of Sciences, Beijing 100049, China,Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;University of Chinese Academy of Sciences, Beijing 100049, China,Center for Environmental Remediation, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;University of Chinese Academy of Sciences, Beijing 100049, China,Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;University of Chinese Academy of Sciences, Beijing 100049, China,Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;University of Chinese Academy of Sciences, Beijing 100049, China,Jiangxi Provincial Key Laboratory for Bamboo Germplasm Resources and Utilization, Jiangxi Agricultural University, Nanchang 330045, China and Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | |
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| Keywords: | nitrogen and phosphorus additions soil carbon and nitrogen mineralization enzyme kinetics Hydrolase subtropical red soil |
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