非培养方法解析北京地区甘薯叶际细菌的群落结构

利用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术和磷脂脂肪酸(PLFA)分析方法,比较了北京通州、顺义、昌平、延庆地区甘薯叶际细菌的多样性和生物量,并调查了通州地区甘薯叶际细菌群落在不同生长季节的变化情况。PLFA分析结果发现所有检测样品中,革兰氏阳性细菌生物量均高于革兰氏阴性细菌生物量。PCR-DGGE方法与PLFA方法聚类分析结果较一致,甘薯叶际细菌群落结构受到时空因素、甘薯生理特性等的影响,不同地点、不同生长季节甘薯叶际细菌群落结构有较大差异,DGGE条带测序分析表明,Pseudomonas sp.在不同地点的甘薯叶际均为保守菌群,Bacillus sp.,Acinetobacter sp.,

Investigation of bacterial community structure within the sweet potato phyllosphere using culture\|independent techniques

Investigation of the variations in the bacterial community on sweet potato leaves over multiple time and space scales could provide important information to describe the identity and the ecology of leaf inhabitants. In this study, bacterial flora in the phyllosphere of sweet potato under field conditions was identified using polymerase chain reaction\|denaturing gradient gel electrophoresis (PCR\|DGGE) and phospholipid fatty acid (PLFA) analysis methods on June 15th, in Shunyi, Tongzhou, Changping and Yanqing, Beijing, China. Furthermore, the bacterial flora in the sweet potato phyllosphere in Tongzhou was investigated during the period of May\|August. PLFA assay indicated that bactieral poplulaition of the gram\|positive bacteria were found to be more than the gram\|negative bacteria in all samples. DGGE bands sequencing showed that Pseudomonas sp. was a persistent bacterium in all samples from different places; Bacillus sp., Acinetobacter sp., Weissella sp., Pantoea sp., Bradyrhizobium sp. and Enterococcus sp. were also detected as the dominant strains within sweet potato phyllosphere under different conditions.