In Situ Fluorescence Imaging of Myelination |
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Authors: | Changning Wang Daniela C Popescu Chunying Wu Junqing Zhu Wendy Macklin Yanming Wang |
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Institution: | Division of Radiopharmaceutical Science, Case Center for Imaging Research, Department of Radiology (CWang,CWu,JZ,YW) and Department of Neurosciences (DCP), Case Western Reserve University, Cleveland, Ohio, and Department of Cell and Developmental Biology, University of Colorado Denver Health Sciences, Aurora, Colorado (WM) |
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Abstract: | We describe a novel fluorescent dye, 3-(4-aminophenyl)-2H-chromen-2-one (termed case myelin compound or CMC), that can be used for in situ fluorescent imaging of myelin in the vertebrate nervous system. When administered via intravenous injection into the tail vein, CMC selectively stained large bundles of myelinated fibers in both the central nervous system (CNS) and the peripheral nervous system (PNS). In the CNS, CMC readily entered the brain and selectively localized in myelinated regions such as the corpus callosum and cerebellum. CMC also selectively stained myelinated nerves in the PNS. The staining patterns of CMC in a hypermyelinated mouse model were consistent with immunohistochemical staining. Similar to immunohistochemical staining, CMC selectively bound to myelin sheaths present in the white matter tracts. Unlike CMC, conventional antibody staining for myelin basic protein also stained oligodendrocyte cytoplasm in the striatum as well as granule layers in the cerebellum. In vivo application of CMC was also demonstrated by fluorescence imaging of myelinated nerves in the PNS. (J Histochem Cytochem 58:611–621, 2010) |
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Keywords: | myelin staining demyelination remyelination coumarin imaging multiple sclerosis PNS nerves 3-(4-aminophenyl)-2H-chromen-2-one |
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