Differential control of Eg5-dependent centrosome separation by Plk1 and Cdk1 |
| |
Authors: | Smith Ewan Hégarat Nadia Vesely Clare Roseboom Isaac Larch Chris Streicher Hansjörg Straatman Kornelis Flynn Helen Skehel Mark Hirota Toru Kuriyama Ryoko Hochegger Helfrid |
| |
Institution: | Genome Damage and Stability Centre, University of Sussex, Brighton, UK. |
| |
Abstract: | Cyclin-dependent kinase 1 (Cdk1) is thought to trigger centrosome separation in late G2 phase by phosphorylating the motor protein Eg5 at Thr927. However, the precise control mechanism of centrosome separation remains to be understood. Here, we report that in G2 phase polo-like kinase 1 (Plk1) can trigger centrosome separation independently of Cdk1. We find that Plk1 is required for both C-Nap1 displacement and for Eg5 localization on the centrosome. Moreover, Cdk2 compensates for Cdk1, and phosphorylates Eg5 at Thr927. Nevertheless, Plk1-driven centrosome separation is slow and staggering, while Cdk1 triggers fast movement of the centrosomes. We find that actin-dependent Eg5-opposing forces slow down separation in G2 phase. Strikingly, actin depolymerization, as well as destabilization of interphase microtubules (MTs), is sufficient to remove this obstruction and to speed up Plk1-dependent separation. Conversely, MT stabilization in mitosis slows down Cdk1-dependent centrosome movement. Our findings implicate the modulation of MT stability in G2 and M phase as a regulatory element in the control of centrosome separation. |
| |
Keywords: | actin Eg5 microtubules mitosis Polo kinase |
本文献已被 PubMed 等数据库收录! |
|