Phospholipase D1: a key factor for the exocytotic machinery in neuroendocrine cells |
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Authors: | Vitale N Caumont A S Chasserot-Golaz S Du G Wu S Sciorra V A Morris A J Frohman M A Bader M F |
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Institution: | CNRS-UPR 2356 Neurotransmission et Sécrétion Neuroendocrine, 5 rue Blaise Pascal, 67084 Strasbourg, France. |
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Abstract: | Phospholipase D (PLD) has been proposed to mediate cytoskeletal remodeling and vesicular trafficking along the secretory pathway. We recently described the activation of an ADP ribosylation factor-regulated PLD at the plasma membrane of chromaffin cells undergoing secretagogue-stimulated exocytosis. We show here that the isoform involved is PLD1b, and, using a real-time assay for individual cells, that PLD activation and exocytosis are closely correlated. Moreover, overexpressed PLD1, but not PLD2, increases stimulated exocytosis in a phosphatidylinositol 4,5-bisphosphate-dependent manner, whereas catalytically inactive PLD1 inhibits it. These results provide the first direct evidence that PLD1 is an important component of the exocytotic machinery in neuroendocrine cells. |
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