APC/CCdh1 controls CtIP stability during the cell cycle and in response to DNA damage |
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| Authors: | Elisabeth GE de Vries Shao‐En Ong Alessandro A Sartori Marcel ATM van Vugt |
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| Institution: | 1. Department of Medical Oncology, University Medical Center Groningen, University of Groningen, , Groningen, The Netherlands;2. Department of Pharmacology, University of Washington, , Seattle, WA, USA;3. Institute of Molecular Cancer Research, University of Zurich, , Zurich, Switzerland |
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| Abstract: | Human cells have evolved elaborate mechanisms for responding to DNA damage to maintain genome stability and prevent carcinogenesis. For instance, the cell cycle can be arrested at different stages to allow time for DNA repair. The APC/CCdh1 ubiquitin ligase mainly regulates mitotic exit but is also implicated in the DNA damage‐induced G2 arrest. However, it is currently unknown whether APC/CCdh1 also contributes to DNA repair. Here, we show that Cdh1 depletion causes increased levels of genomic instability and enhanced sensitivity to DNA‐damaging agents. Using an integrated proteomics and bioinformatics approach, we identify CtIP, a DNA‐end resection factor, as a novel APC/CCdh1 target. CtIP interacts with Cdh1 through a conserved KEN box, mutation of which impedes ubiquitylation and downregulation of CtIP both during G1 and after DNA damage in G2. Finally, we find that abrogating the CtIP–Cdh1 interaction results in delayed CtIP clearance from DNA damage foci, increased DNA‐end resection, and reduced homologous recombination efficiency. Combined, our results highlight the impact of APC/CCdh1 on the maintenance of genome integrity and show that this is, at least partially, achieved by controlling CtIP stability in a cell cycle‐ and DNA damage‐dependent manner. |
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| Keywords: | Cdh1 cell cycle CtIP DNA damage DNA double‐strand break repair |
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