Structural studies of the O-antigen polysaccharides of Klebsiella O5 and Escherichia coli O8 |
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| Authors: | Per-Erik Jansson Jörgen Lönngren Göran Widmalm Karin Leontein Kerstin Slettengren Stefan B Svenson Göran Wrangsell Anne Dell Philip R Tiller |
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| Institution: | Department of Organic Chemistry, Arrhenius Laboratory, University of Stockholm, S-106 91 Stockholm Sweden;Department of Bacteriology, National Bacteriological Laboratory, S-105 21 Stockholm Sweden;Department of Biochemistry, Imperial College of Science and Technology, Imperial Institute Road, London SW7 2AZ Great Britain |
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| Abstract: | The O-antigen polysaccharides of Klebsiella serotype O5 and Escherichia coli serotype O8 are serologically very similar or identical. The structures of these two polysaccharides have now been re-investigated. N.m.r. spectroscopy, chromium trioxide oxidation, hydrolysis with a specific phage enzyme, and f.a.b. mass spectrometry were the principal methods used. It is concluded that the O-antigen has the following structure, in which D-Man3Me is 3-O-methyl-D-mannose and n is approximately 10. (Formula: see text) Biosynthetic studies indicate that these antigens are synthesised by addition of D-mannopyranosyl groups to the "non-reducing" end of the mannan chain, and it seems possible that addition of a 3-O-methyl-D-mannopyranosyl group involves termination. |
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