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Structural analysis of the O-polysaccharide from the lipopolysaccharide of Azospirillum brasilense S17 |
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| Authors: | Fedonenko Yuliya P Konnova Olga N Zdorovenko Evelina L Konnova Svetlana A Zatonsky George V Shashkov Alexander S Ignatov Vladimir V Knirel Yuriy A |
| Institution: | Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, 410049 Saratov, Russia. |
| Abstract: | A mixture of two structurally distinct neutral O-polysaccharides was obtained by mild acid degradation of the lipopolysaccharide isolated by the phenol/water extraction from the asymbiotic diazotrophic rhizobacterium Azospirillum brasilense S17. The following structures of the O-polysaccharides were established by composition and methylation analyses, Smith degradation, and 1H and 13C NMR spectroscopy, including a 2D NOESY experiment: Formula: see text] where L-Rha2Me stands for 2-O-methyl-L-rhamnose and SHb for the (S)-3-hydroxybutanoyl group. The occurrence of two distinct polysaccharides is reported for the first time in Azospirillum spp. |
| Keywords: | Lipopolysaccharide Bacterial polysaccharide structure Azospirillum brasilense l-rhamnose" target="_blank">2-O-Methyl-l-rhamnose |
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