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Preparation of chitooligosaccharides from chitosan by a complex enzyme |
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| Authors: | Zhang H Du Y Yu X Mitsutomi M Aiba S |
| Institution: | a Department of Biochemical Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian 116023, People's Republic of China b Department of Applied Biological Sciences, Saga University, Saga 840, Japan c Functional Polymer Section, Osaka National Research Institute, Osaka 653, Japan |
| Abstract: | Chitosan of 24% degree of acetylation was depolymerized by a mixture of cellulase, alpha amylase, and proteinase to give the title oligosaccharides. The removal of products by membrane separation permitted yield maximization of products having degree of polymerization in the 3–10 range. |
| Keywords: | Chitooligosaccharides Enzyme mixture Hydrolysis Chitosan |
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