Isolation and characterization of a mannoglucan from edible Cordyceps sinensis mycelium |
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Authors: | Wu Yalin Hu Nan Pan Yuanjiang Zhou Lijun Zhou Xuxia |
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Institution: | Department of Chemistry, Zhejiang University, Hangzhou 310027, People's Republic of China. |
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Abstract: | Cordyceps sinensis is a well known tonic food or invigorant with broad-spectrum medicinal properties that is widely used in the People's Republic of China. A neutral mannoglucan 1 with a molar mass of approximately 7.7x10(3) Da was obtained from the 0.05 M acetate buffer extract of C. sinensis mycelium. It had alpha](D)(20)+126 (c 0.2, H(2)O) and consisted of Man and Glc units in the molar ratio of 1:9. A combination of chemical analysis and NMR and IR spectroscopy together with digestion with alpha-D-amylase showed 1 to have a alpha-D-glucan backbone with (1-->4)- and (1-->3)-linkages, and the side chains of alpha-D-(1-->6)-Manp were attached to the backbone via O-6 of alpha-(1-->3)-Glcp residues. Compound 1 showed weak cytotoxicity activity against SPC-I (IC(50)=63 microg/mL) cancer line, and no obvious cytotoxicity activities against BCAP37 (IC(50)>100 microg/mL) and SW480 (IC(50)>100 microg/mL) cancer lines. |
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Keywords: | Cordyceps sinensis Mannoglucan Methylation Periodate and chromium trioxide oxidation NMR and IR spectroscopy Antitumor activity |
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