Studies of the molecular recognition of synthetic methyl beta-lactoside analogues by Ricinus communis agglutinin. |
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| Authors: | A Rivera-Sagredo J Jiménez-Barbero M Martín-Lomas D Solís T Díaz-Mauri?o |
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| Institution: | Instituto de Química Orgánica, C.S.I.C., Madrid, Spain. |
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| Abstract: | The 2-, 3-, 6-, 2'-, 3'-, 4'-, and 6'-deoxy derivatives and the 3-O-methyl derivative of methyl beta-lactoside have been synthesised and their binding to the galactose-specific agglutinin from Ricinus communis (RCA-120) has been investigated. The results indicate that HO-3,4,6 of the beta-D-galactopyranose moiety are the key polar groups. The main difference from the closely related ricin lectin RCA-60 involves HO-6 of the D-glucopyranose moiety, which seems to contribute to the binding of the carbohydrate to RCA-60 but not to RCA-120. |
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