Synthesis of Highly Immunogenic Multiple Antigenic Peptides for Epitopes of Viral Antigen to Use in ELISA |
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Authors: | Paramasivam Saravanan Sameer Shrivastava Satish Kumar |
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Institution: | (1) National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243 122, India;(2) Animal Biotechnology, FMD Vaccine Centre, Indian Veterinary Research Institute Campus, Hebbal, Bangalore, Karnataka, 560 024, India |
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Abstract: | Synthesis of multiple antigenic peptides (MAPs) for predicted antigenic determinants of a viral antigen is described. The
method includes prediction of linear epitopes using predictive computer algorithms, synthesis of peptides for the predicted
regions, testing of peptides to find the most reactive sites, synthesis of MAPs and their testing. The procedure involves
manual synthesis of MAPs by solid phase peptide synthesis with Wang resin as solid support. The MAPs were prepared in eight
copies and used for immunization of rabbits to generate anti-peptide antibodies. Further, the reactivity of MAPs in detecting
the native cognate antigen in the whole virus was confirmed by ELISA. The MAP and anti-peptide antibodies could serve as diagnostic
tools for viral diseases. MAPs have efficiently been used to confirm the presence of linear antigenic and immunogenic epitopes
on viral proteins, for possible use in diagnostic and vaccine. It was suggested that this method could help in epitope mapping
of dreadful human or animal pathogens as it involves production of safe, chemically defined and non-infectious materials for
use as antigen as well as immunogen. |
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