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Nanoelectromechanics of Methylated DNA in a Synthetic Nanopore
Authors:U Mirsaidov  X Zou  V Dimitrov  AP Feinberg
Institution: Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois
School of Medicine, The Johns Hopkins University, Baltimore, Maryland
§ School of Physics, Peking University, Beijing, China
Abstract:Methylation of cytosine is a covalent modification of DNA that can be used to silence genes, orchestrating a myriad of biological processes including cancer. We have discovered that a synthetic nanopore in a membrane comparable in thickness to a protein binding site can be used to detect methylation. We observe a voltage threshold for permeation of methylated DNA through a <2 nm diameter pore, which we attribute to the stretching transition; this can differ by >1 V/20 nm depending on the methylation level, but not the DNA sequence.
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