Overexpression of the (R)-specific enoyl-CoA hydratase gene from Pseudomonas chlororaphis HS21 in Pseudomonas strains for the biosynthesis of polyhydroxyalkanoates of altered monomer composition

The (R)-specific enoyl-CoA hydratase gene (phaJ(HS21)) from Pseudomonas chlororaphis HS21 was overexpressed in various Pseudomonas strains, alone and in combination with the polyhydroxyalkanoate synthase gene (phaC(HS21)), for the biosynthesis of polyhydroxyalkanoates (PHAs) of altered monomer composition. Recombinant Pseudomonas strains harboring phaC(HS21) and phaJ(HS21) generated saturated and unsaturated monomers of C12-C14 in their PHAs. In particular, the level of the 3-hydroxytetradecenoate monomer in recombinant P. chlororaphis HS21 increased by approximately 260%. PhaJ(HS21) is expected to be useful in the biosynthesis of PHAs consisting of unusual monomer units.