Chitinous components of the cell wall of Fusarium oxysporum. |
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Authors: | T Fukamizo T Ohkawa K Sonoda H Toyoda T Nishiguchi S Ouchi S Goto |
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Institution: | Laboratory of Biophysical Chemistry, Faculty of Agriculture, Kinki University, Nara, Japan. |
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Abstract: | The cell wall of Fusarium oxysporum f. sp. lycopersici was digested with chitinase to analyze the structure of its chitinous components. In spite of a similar acetylation degree of the cell wall components to that of 25-35% acetylated chitosan, only N-acetylglucosamine disaccharide (GlcNAc)2] was obtained from chitinase hydrolyzate of the fungal cell wall by CM-Sephadex C-25 column chromatography, while (GlcNAc)2 and several types of deacetylated chitooligosaccharides were separated from that of 25-35% acetylated chitosan. The results indicate that N-acetylglucosamine residues in the polysaccharide chains of the fungal cell wall are most likely condensed into some region, while acetylated residues are more scattered in 25-35% acetylated chitosan. |
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