Gene encoding a trehalose phosphorylase from Thermoanaerobacter brockii ATCC 35047 |
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| Authors: | Maruta Kazuhiko Mukai Kazuhisa Yamashita Hiroshi Kubota Michio Chaen Hiroto Fukuda Shigeharu Kurimoto Masashi |
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| Institution: | Amase Institute, Hayashibara Biochemical Laboratories, Inc, Okayama, Japan. amaseken@hayashibara.co.jp |
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| Abstract: | A gene encoding a trehalose phosphorylase was cloned from Thermoanaerobacter brockii ATCC 35047. The gene encodes a polypeptide of 774 amino acid residues. The deduced amino acid sequence was homologous to bacterial maltose phosphorylases and a trehalose 6-phosphate phosphorylase catalyzing anomer-inverting reactions. On the other hand, no homology was found between the T. brockii enzyme and an anomer-retaining trehalose phosphorylase from Grifola frondosa. |
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