Comprehensive DNA microarray expression profiles of tumors in tenascin-C-knockout mice |
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| Authors: | Kaori Matsumoto Yuji Nakai Masaru Hoshino Koki Yamazaki Yoshiaki Takioto Satoru Takadera |
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| Institution: | 1. Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan;2. Institute for Food Sciences, Hirosaki University, Aomori, Japan;3. Advanced Technology Research Laboratory, Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan |
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| Abstract: | Tenascin-C (TNC), an extracellular matrix glycoprotein, plays a pivotal role in tumor growth. However, the mechanism whereby TNC affects tumor biology remains unclear. To investigate the exact role of TNC in primary tumor growth, a mouse mammary tumor cell line, GLMT1, was first developed. Subsequently, global gene expression in GLMT1-derived tumors was compared between wild-type (WT) and TNC-knockout (TNKO) mice. Tumors in WT mice were significantly larger than those in TNKO mice. DNA microarray analysis revealed 447 up and 667 downregulated in the tumors inoculated into TNKO mice as compared to tumors in WT mice. Validation by quantitative gene expression analysis showed that Tnc, Cxcl1, Cxcl2, and Cxcr2 were significantly upregulated in WT mice. We hypothesize that TNC stimulates the CXCL1/2-CXCR2 pathway involved in cancer cell proliferation. |
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| Keywords: | tenascin-C tumor growth DNA microarray gene expression angiogenesis |
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