The effects of Bombyx mori silk strain and extraction time on the molecular and biological characteristics of sericin |
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| Authors: | Tippawan Siritientong Walter Bonani Antonella Motta Claudio Migliaresi |
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| Institution: | 1. Faculty of Pharmaceutical Sciences, Bioactive Resources for Innovative Clinical Applications Research Unit and Department of Pharmacy Practice, Chulalongkorn University, Bangkok, Thailand;2. Department of Industrial Engineering and BIOtech Research Centre, University of Trento, Trento, Italy;3. European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Trento, Italy;4. INSTM Research Unit, Trento Research Unit, Trento, Italy |
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| Abstract: | Sericin was extracted from three strains of Thai Bombyx mori silk cocoons (white shell Chul1/1, greenish shell Chul3/2, and yellow shell Chul4/2) by a high-pressure and high-temperature technique. The characteristics of sericin extracted from different fractions (15, 45, and 60 min extraction process) were compared. No differences in amino acid composition were observed among the three fractions. For all silk strains, sericin extracted from a 15-min process presented the highest molecular weight. The biological potential of the different sericin samples as a bioadditive for 3T3 fibroblast cells was assessed. When comparing sericin extracted from three silk strains, sericin fractions extracted from Chul4/2 improved cell proliferation, while sericin from Chul 1/1 activated Type I collagen production to the highest extent. This study allows the natural variability of sericin obtained from different sources and extraction conditions to be addressed and provides clues for the selection of sericin sources. |
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| Keywords: | sericin extraction amino acid molecular weight biological properties |
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