A colony filter-hybridization procedure for the filamentous fungus Neurospora crassa |
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| Authors: | L L Stohl A M Lambowitz |
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| Institution: | Edward A. Doisy Department of Biochemistry, St. Louis University School of Medicine, St. Louis, Missouri, 63104 USA |
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| Abstract: | A colony filter-hybridization procedure for the filamentous fungus Neurospora crassa has been developed. The procedure is sensitive enough to detect Escherichia coli plasmid pBR322 DNA integrated into chromosomal DNA in a Neurospora transformant. Thus, it should facilitate the isolation of nuclear genes by plasmid-rescue procedures. |
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| Keywords: | colony filter-hybridization transformation gene isolation |
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