Simultaneous electrochemical detection of multiple biomarkers using gold nanoparticles decorated multiwall carbon nanotubes as signal enhancers |
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Authors: | Dexiang Feng Lihua Li Junqing Zhao Yuzhong Zhang |
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Institution: | 1. College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, People’s Republic of China;2. Department of Chemistry, Wannan Medical College, Wuhu 241002, People’s Republic of China |
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Abstract: | In this work, a novel sandwich-type electrochemical immunosensor has been developed for simultaneous detection of carcinoembryonic antigen (CEA) and α-fetoprotein (AFP) based on metal ion labels. Gold nanoparticles decorated multiwall carbon nanotubes (AuNPs@MWCNTs) were used as carriers to immobilize secondary antibodies and distinguishable electrochemical tags of Pb2+ and Cd2+ to amplify the signals. Due to the intrinsic property of high surface-to-volume ratio, the AuNPs@MWCNTs could load numerous secondary antibodies and labels. Therefore, the multiplexed immunoassay exhibited good sensitivity and selectivity. Experimental results revealed that this sandwich-type immunoassay displayed an excellent linear response, with a linear range of 0.01 to 60 ng mL–1 for both analytes and detection limits of 3.0 pg mL–1 for CEA and 4.5 pg mL–1 for AFP (at a signal-to-noise ratio of 3). The method was successfully applied for the determination of AFP and CEA levels in clinical serum samples. |
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Keywords: | Gold nanoparticles decorated multiwall carbon nanotubes Pb2+ and Cd2+ Multiplex assay Carcinoembryonic antigen α-Fetoprotein |
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