Improved continuous-flow print head for micro-array deposition |
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| Authors: | Eddings Mark A Miles Adam R Eckman Josh W Kim Jungkyu Rich Rebecca L Gale Bruce K Myszka David G |
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| Institution: | aDepartment of Bioengineering, University of Utah, Salt Lake City, UT 84132, USA;bWasatch Microfluidics, North Salt Lake City, UT 84054, USA;cCenter for Biomolecular Interaction Analysis, University of Utah, Salt Lake City, UT 84112, USA;dDepartment of Mechanical Engineering, University of Utah, Salt Lake City, UT 84132, USA |
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| Abstract: | Limitations in depositing ligands using conventional micro-array pin spotting have hindered the application of surface plasmon resonance imaging (SPRi) technology. To address these challenges we introduce a modification to our continuous-flow micro-spotting technology that improves ligand deposition. Using Flexchip™ protein A/G and neutravidin capturing surfaces, we demonstrate that our new microfluidic spotter requires 1000 times less concentrated antibodies and biotinylated ligands than is required for pin spotting. By varying the deposition flow rate, we show that the design of our tip overlay flow cell is efficient at delivering sample to the substrate surface. Finally, contact time studies show that it is possible to capture antibodies and biotinylated ligands at concentrations of less than 0.1 ug/ml and 100 pM, respectively. These improvements in spotting technology will help to expand the applications of SPRi systems in areas such as antibody screening, carbohydrate arrays, and biomarker detection. |
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| Keywords: | Biacore Flexchip Protein arrays |
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