Selective removal of keratan sulfate in chondroitin sulfate samples by sequential precipitation with ethanol |
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Authors: | Fabio Galeotti Francesca MaccariNicola Volpi |
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Institution: | Department of Life Sciences, University of Modena and Reggio Emilia, 41100 Modena, Italy |
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Abstract: | Keratan sulfate (KS) is present as a contaminant in chondroitin sulfate (CS) mainly extracted from shark cartilage. We report a selective removal procedure of KS in CS samples by means of sequential precipitation with ethanol. Purified shark CS containing approximately 10% to 15% KS was subjected to a precipitation procedure in the presence of increasing percentages of saturated ethanol. In contrast to other solvents, 1.0 volume of ethanol was able to selectively purify CS, with a purity of approximately 100%, from KS. The current selective and simple procedure appears to be a reliable industrial preparation of CS devoid of large amounts of the residual KS. |
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Keywords: | Chondroitin sulfate Keratan sulfate Ethanol precipitation Hexosamines Capillary electrophoresis Glycosaminoglycans |
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