Label-free fluorescent sensor for lead ion detection based on lead(II)-stabilized G-quadruplex formation |
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Authors: | Shenshan Zhan Yuangen WuYanfang Luo Le LiuLan He Haibo XingPei Zhou |
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Institution: | School of Environmental Science and Engineering, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, School of Agriculture and Biology, and Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of China |
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Abstract: | A label-free fluorescent DNA sensor for the detection of lead ions (Pb2+) based on lead(II)-stabilized G-quadruplex formation is proposed in this article. A guanine (G)-rich oligonucleotide, T30695, was used as a recognition probe, and a DNA intercalator, SYBR Green I (SG), was used as a signal reporter. In the absence of Pb2+, the SG intercalated with the single-stranded random-coil T30695 and emitted strong fluorescence. While in the presence of Pb2+, the random-coil T30695 would fold into a G-quadruplex structure and the SG could barely show weak fluorescence, and the fluorescence intensity was inversely proportional to the involving amount of Pb2+. Based on this, a selective lead ion sensor with a limit of detection of 3.79 ppb (parts per billion) and a detection range from 0 to 600 ppb was constructed. Because detection for real samples was also demonstrated to be reliable, this simple, low-cost, sensitive, and selective sensor holds good potential for Pb2+ detection in real environmental samples. |
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Keywords: | Fluorescent sensor Lead ion detection G-quadruplex |
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